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| 不同基因型大豆GS1基因的克隆与分析 | |
| 引用本文： | 陈丽华,刘丽君,刘页丽,裴宇峰,祖伟.不同基因型大豆GS1基因的克隆与分析[J].东北农业大学学报,2011,42(7):101-106. |
| 作者姓名： | 陈丽华 刘丽君 刘页丽 裴宇峰 祖伟 |
| 作者单位： | 1. 国家农业标准化监测与研究中心,哈尔滨,150036 2. 东北农业大学农学院,哈尔滨,150030 3. 黑龙江农垦科研育种中心,哈尔滨,150090 |
| 基金项目： | 国家“十一五”科技支撑计划资助项目，黑龙江省教育厅科研项目 |
| 摘 要： | 克隆了6个不同基因型大豆的GS1基因,序列比对分析结果表明,不同基因型大豆的GS1基因序列相似性较高.不同基因型大豆GS1基因序列间仍存在显著的差异,主要集中在非编码区.GS1基因分子进化树显示东农42、半野生大豆基因序列进化距离较近.不同基因型大豆GS1蛋白质序列具有很高的相似性,并且都具有两个保守的结构域、催化结构... |
| 关 键 词： | 大豆 GS 基因 克隆 |
Cloning and analysis of GS1 genes from different soybean varieties |
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| CHEN Lihua,LIU Lijur,LIU Yeli,PEI Yufeng,ZU Wei.Cloning and analysis of GS1 genes from different soybean varieties[J].Journal of Northeast Agricultural University,2011,42(7):101-106. | |
| Authors: | CHEN Lihua LIU Lijur LIU Yeli PEI Yufeng ZU Wei |
| Institution: | 1.National Agriculture Standardize Supervise and Research Center,Harbin 150036,China;2.College of Agriculture,Northeast Agricultural University,Harbin 150030,China;3.Land Reclamation Research and Breeding Centre of Heilongjiang,Harbin 150090,China) |
| Abstract: | GS1 genes from six different soybean varieties were cloned.The compare analysis of gene sequence showed that: sequence of GS1 genes from different soybean varieties were very identical.GS1 gene sequence from different soybean varieties still existed distinct difference which site mostly in non-coding region.Molecular evolutionary tree showed that evolution distance of GS1 genes sequence from DN42 and G.gracili were relatively near,they all had two conserved domains,catalyse domain(Gln-synt_N)and combine domain(Gln-synt_C),and showed that they all had function of catalyse.Molecular evolution tree showed that evolution distance of GS1 protein sequence of DN42 and DN46 was near,KF9 and HN35 was near.GS1 genome sequence of DN42 was cloned.The forecast result through NetGene2 software,Dot metrix function of DNAMan software and NCBI online spidey software showed that this gene had 12 exons and 11 introns,the flank sequence of exon were all accord with GT,AG rule. |
| Keywords: | soybean GS1 genes clone |
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