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猪增生性肠炎黏膜免疫相关细胞数量研究
引用本文:陈彤,杨小燕,祁保民.猪增生性肠炎黏膜免疫相关细胞数量研究[J].东北农业大学学报,2011,42(12):25-29.
作者姓名:陈彤  杨小燕  祁保民
作者单位:1. 龙岩学院生命科学学院,福建龙岩364000;福建省人畜寄生与病毒性疫病防控工程技术研究中心,福建龙岩364000
2. 福建农林大学动物科学学院,福州,350002
基金项目:福建省区域科技重大项目
摘    要:为了揭示猪增生性肠炎黏膜免疫相关细胞数量的变化情况.采用组织学方法,比较PCR检验阳性猪(获得胞内劳森氏菌特异性扩增产物)与阴性猪(无胞内劳森氏菌特异性扩增产物)的十二指肠、空肠、回肠黏膜淋巴细胞、杯状细胞、肥大细胞的数量和分布变化.结果表明,阳性猪十二指肠、空肠黏膜上皮内每100个柱状细胞间淋巴细胞的数量同比阴性猪稍...

关 键 词:  猪增生性肠炎  小肠  淋巴细胞  杯状细胞  肥大细胞

Research on the number of mucosal immunity-associated cell in porcine proliferative enteritis
CHEN Tong,YANG Xiaoyan,QI Baomin.Research on the number of mucosal immunity-associated cell in porcine proliferative enteritis[J].Journal of Northeast Agricultural University,2011,42(12):25-29.
Authors:CHEN Tong  YANG Xiaoyan  QI Baomin
Abstract:In order to reveal change of the number of mucosal immunity-associated cell in porcine proliferative enteritis.Using light microscope method to compare the number and distribution change of duodenum,jejunum,ileum mucous membrane lymphocyte,goblet cell,and mast cell of the positive swine(with Lawsonia intracellularis specificity amplified products) and the negative swine(without Lawsonia intracellularis specificity amplified products)after the PCR test.The results showed that: In duodenum and jejunum mucous membrane epithelium cell of the positive swine,compared with the negative swine,lymphocyte's number in each 100 columnar cells decreased slightly(P>0.05),ileum the same increased slightly(P>0.05);In duodenum,jejunum and ileum mucous membrane epithelium cell of the positive swine,compared with the negative swine,goblet cell's number in each 100 columnar cells increased significantly(P<0.05);Mast cell's number in mucosal lamina propria of the jejunum and ileum in positive swine,compared with the negative swine,increased significantly(P<0.05).The research helps to understand the status of intestinal mucosa′s immunological barrier function in porcine proliferative enteritis and provides a reference for the prevention and treatment of the disease.
Keywords:pig  porcine proliferative enteritis  small intestine  lymphocyte  goblet cell  mast cell
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