| 培养基添加物对马铃薯花药褐化及愈伤诱导的影响 |
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| 引用本文： | 赵欣,卢翠华,陈伊里,石瑛,邸宏,张丽莉,张正国,张欢.培养基添加物对马铃薯花药褐化及愈伤诱导的影响[J].东北农业大学学报,2010,41(1). |
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| 作者姓名： | 赵欣 卢翠华 陈伊里 石瑛 邸宏 张丽莉 张正国 张欢 |
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| 作者单位： | 东北农业大学农学院,哈尔滨,150030;东北农业大学农学院,哈尔滨,150030;东北农业大学农学院,哈尔滨,150030;东北农业大学农学院,哈尔滨,150030;东北农业大学农学院,哈尔滨,150030;东北农业大学农学院,哈尔滨,150030;东北农业大学农学院,哈尔滨,150030;东北农业大学农学院,哈尔滨,150030 |
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| 基金项目： | 黑龙江省科技攻关计划项目(GB07B104) |
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| 摘 要： | 试验以Vangal、波C、讷16、春薯4号和内薯7号5个马铃薯品种(系)为材料,研究不同培养基添加物硝酸银、活性炭、马铃薯提取液对马铃薯花药培养的褐化率和愈伤诱导率的影响。结果表明,培养基中加入20mg·L-1硝酸银、0.5g·L-1活性炭和50g·L-1马铃薯提取液能够提高马铃薯花药愈伤诱导率,同时褐化率明显降低。
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| 关 键 词： | 马铃薯 花药培养 褐化 愈伤诱导 |
Effect of supplement for culture media on callus browning and callus formation in potato anther culture |
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| ZHAO Xin,LU Cuihua,CHEN Yili,SHI Ying,DI Hong,ZHANG Lili,ZHANG Zhengguo,ZHANG Huan.Effect of supplement for culture media on callus browning and callus formation in potato anther culture[J].Journal of Northeast Agricultural University,2010,41(1). |
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| Authors: | ZHAO Xin LU Cuihua CHEN Yili SHI Ying DI Hong ZHANG Lili ZHANG Zhengguo ZHANG Huan |
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| Institution: | College of Agriculture;Northeast Agricultural University;Harbin 150030;China |
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| Abstract: | Factors that influence callus induction and anther browning rate, such as AgNO_3 concentra-tion, active carbon and potato extract during the culture process were investigated by using five genotypes of Vangal, Bo C, Ne 16, Chunshu 4 and Neishu 7. The results showed that the medium adding 20 mg· L~(-1)AgNO_3.
0.5 g· L~(-1) activate carbon and 50 g· L~(-1) potato extract could improve the rate of callus induction, at the same time, it could reduce the browning rate. |
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| Keywords: | potato anther culture browning callus induction |
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