ISSR和ITS分子标记在黑龙江省野生黑木耳遗传多样性上的应用

利用ISSR和ITS标记对黑龙江省采集的33株野生黑木耳菌株和8个黑龙江省常见栽培菌株进行遗传多样性分析,利用PCR-ISSR体系,选出9个ISSR引物对菌株DNA进行扩增,通过聚类分析对遗传多样性进行分析。结果表明,9个ISSR引物扩增条带103条条带,其中多态性条带95条,多态性比率为92.2%,平均每对引物扩增出条带11.4条,平均每对引物扩增出多态性条带10.6条,平均多态性比例为91.7%。多态性信息含量(PIC)变化在0.063~0.924之间。通过ITS序列对黑木耳菌株进行亲缘关系分析,利用软件ClustalX 1.83和MAGA 4.1进行系统发育分析分析。结果表明,黑木耳ITS1、5.8S、ITS2三个区信息为点比例达到52.1%,遗传位点丰富。两种标记方法均显示黑木耳野生菌株间遗传多样性优于栽培菌株。ISSR和ITS两种方法联合使用可得到较好结果。

Application of ISSR and ITS molecular markers in genetic diversity of Heilongjiang Province wild Auricularia auricula

A genetic diversity analysis of 33 wild Auricularia auricula strains and 8 cultivating strains by using ISSR and ITS markers.Using PCR-SRAP system,we selected 9 pairs of ISSR primers to amplify the DNA of those strains and then analysed genetic diversity by clustering analysis.The results showed: 103 fragments were amplified;Polymorphic bands were 95 which accounted for 92.2% in the total amplified fragments.The number of amplified and polymorphic fragments was 11.4 and 10.6 per primer pair which averaging accounted for 91.7% in the total amplified fragments.The PIC(polymorphism information content) value of these ITS markers varied from 0.063 to 0.924.Analysed the genetic relationship of Auricularia auricula strains by their sequences.Did phylogeny analysis by using Software ClustalX 1.83 and MEGA 4.1.The results showed: ITS1,5.8S and ITS2 had genetic loci as high as 52.1% with abundant genetic sites.Both of the two marking methods showed that the genetic diversity of wild Auricularia auricula strains are better than cultivated strains.Good results can be achieved by combine ISSR and ITS