矮牵牛(Petunia hybrida Vilm)组织培养技术研究

实验以矮牵牛(Petunia hybrida Vilm)的花瓣为培养材料,在MS培养基中附加不同浓度的细胞分裂素(6-BA)和生长素(NAA),进行矮牵牛快速繁殖技术研究。结果表明,MS+6-BA(2.0 mg.L-1)+NAA(0.1mg.L-1)培养基愈伤组织发生的较早,数量多,培养基为最佳分化培养基,不定芽发生早,粗壮,数量多,玻璃化程度很小;诱导不定根时,1/2 MS+NAA(0.5 mg.L-1)培养基诱导生根的时间较早,不定根发生率达100%。

Study on the technique system of tissue culture in Petunia hybrida Vilm

This experiment is that Buds of Petunia hybrida Vilm were cultivated in MS culture medium,and adding different kinds and different concentration of CTK and IAA.The results are as follows,on the culture medium of induce callus,CTK and IAA both induce the callus of the explant,the callus were induced earlier and more in MS+6-BA(2.0 mg·L-1)+NAA(0.1 mg·L-1).The best differentiation culture medium is MS + 6-BA(2.0 mg·L-1)+NAA(0.1 mg·L-1),adventitious buds were induced earlier,thicker and more,a fat lot of vitrification.The best culture medium of producing the root is 1/2 MS + NAA(0.5 mg·L-1),the rate of inducing root is highest in the culture medium(100%),and the induced root are more and thicker.