| 大花萱草组织培养研究 |
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| 引用本文： | 刘志洋,李海涛,朱祥春,孟婧,高继国.大花萱草组织培养研究[J].东北农业大学学报,2008,39(1):43-45. |
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| 作者姓名： | 刘志洋 李海涛 朱祥春 孟婧 高继国 |
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| 作者单位： | 1. 哈尔滨市农业科学院,哈尔滨,150070
2. 东北农业大学生命科学学院,哈尔滨,150030 |
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| 基金项目： | 东北农业大学开放实验室基金 |
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| 摘 要： | 采取大花萱草茎尖为外植体接种在不同培养基上,研究了不同浓度的NAA和6-BA对于外植体生长的影响,并探索了最适于产生愈伤组织和生根的培养基配方。结果表明,有利于外植体产生愈伤组织的培养基配方是MS+6-BA1.0mg·L-1+NAA0.05mg·L-1+30g糖+8g琼脂;适合愈伤组织诱导生芽培养基配方是MS+6-BA1.0mg·L-1+30g糖+8g琼脂;适合幼苗生根的培养基配方是1/2MS+NAA0.5mg·L-1+30g糖+8g琼脂。
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| 关 键 词： | 大花萱草 组织培养 愈伤组织 |
| 文章编号： | 1005-9369（2008）01-0043-03 |
| 收稿时间： | 2006-12-21 |
| 修稿时间： | 2006年12月21 |
Study on tissue culture of Hemerocallis fulva |
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| LIU Zhiyang,LI Haitao,ZHU Xiangchun,MENG Jing,GAO Jiguo.Study on tissue culture of Hemerocallis fulva[J].Journal of Northeast Agricultural University,2008,39(1):43-45. |
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| Authors: | LIU Zhiyang LI Haitao ZHU Xiangchun MENG Jing GAO Jiguo |
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| Abstract: | The stem apex of Hemerocallis fulva was taken as explant cultured in vitro to induce callus with different culture medium. The effects of different concentration 6-BA and NAA culture medium on explant growth was studied. The results showed that MS+6-BA 1.0 mg·L-1+NAA 0.05 mg·L-1+30 g sugar+8 g agaragar solid culture medium was advantageous to induce callus;after cultured in MS+6-BA 1.0 mg·L-1+30 g sugar+8 g agar-agar, the compact construction callus was formed and differentiation, the clamp seedling was formed;and 1/2 MS+NAA 0.5 mg·L-1+30 g sugar+8 g agaragar solid culture medium was the best medium to induce root. |
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| Keywords: | Hemerocallis fulva tissue culture callus tissue |
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