| 甘蓝夜蛾核型多角体病毒(M_bNPV)的生物测定 |
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| 引用本文： | 张洪祥,张履鸿.甘蓝夜蛾核型多角体病毒(M_bNPV)的生物测定[J].东北农业大学学报,1988(4). |
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| 作者姓名： | 张洪祥 张履鸿 |
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| 作者单位： | 东北农学院昆虫教研室，东北农学院昆虫教研室 |
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| 摘 要： | 从甘蓝夜蛾(Mamestra brassicae L.)自然罹病死亡幼虫体分离出的多株核型多角体病毒(M_bNPV),选出毒力较强的 M_bNPV-80株,于1984年在哈尔滨进行生物活性测定。方差分析表明:1.2×10~2～1.2×10~8 PIB(多角体)/虫间,7种剂量感染3龄幼虫,不同剂量间的校正死亡率差异极显著。而各处理死亡均数差异显著性测定中,1.2×10~5～1.2×10~8 PIB/虫4个剂量差异不显著。剂量与死亡率回归方程为 y=2.819+0.416x;致死中剂量(LD_(50))为1.77×10~5 PIB/虫。每虫以1.2×10~4和1.2×10~5 PIB/虫分别处理3龄幼虫所得的致死中时(LT_(50))分别为6.59和4.31天。
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| 关 键 词： | 甘蓝夜蛾 病毒 核型多角体病毒 生物测定 |
BIOASSAY OF THE NUCLEAR POLYHEDROSIS VIRUS OF THE CABBAGE MOTH(MAMESTRA BRASSICAE L.) |
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| Zhang Hongxiang Zhang Luhong.BIOASSAY OF THE NUCLEAR POLYHEDROSIS VIRUS OF THE CABBAGE MOTH(MAMESTRA BRASSICAE L.)[J].Journal of Northeast Agricultural University,1988(4). |
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| Authors: | Zhang Hongxiang Zhang Luhong |
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| Institution: | Zhang Hongxiang Zhang Luhong |
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| Abstract: | Bioassay of an isolate of Mamestra brassicae nuclear polyhedrosis virusMbNPV-80 was conducted in Harbin on third instar larvae of cabbage mothwhich were individually reared on cabbage leaf contaminated with the polyhe-dra.The regression equation between dosage and mortality was Y=2.819+0.416X.The LD_(50)value and 95% fiducial limits were 1.77x10~5 PIB/larva and6.62×10~4-4.73×10~5 PIB/larva.The LT_(50)values for 1.2×10~4 and 1.2×10~5 PIB/larva treatments were 6.59 and 4.31 days respectively. |
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| Keywords: | cabbage moth(Mamestra brassicae L ) virus nuclear polyhedrosis virus bioassay |
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