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植物乳杆菌培养基的优化
引用本文:李想,王然,程建军,李琦,王鹏.植物乳杆菌培养基的优化[J].东北农业大学学报,2008,39(9).
作者姓名:李想  王然  程建军  李琦  王鹏
作者单位:东北农业大学食品学院,哈尔滨,150030
基金项目:黑龙江省教育厅科学技术研究项目
摘    要:本研究主要是针对分离自发酵糯玉米的植物乳杆菌,为了促进植物乳杆菌的增殖,对其培养基进行了优化。单因素试验结果表明,最佳碳源、氮源、磷源分别为蔗糖、酵母膏、KH2PO4。正交试验确定的最佳添加量是蔗糖2%、酵母膏2.5%、KH2PO40.2%,菌落总数为3.0×108cfu·mL-1。

关 键 词:植物乳杆菌  培养基  优化

Optimization of culture medium for L. plantarum
LI Xiang,WANG Ran,CHENG Jianjun,LI Qi,WANG Peng.Optimization of culture medium for L. plantarum[J].Journal of Northeast Agricultural University,2008,39(9).
Authors:LI Xiang  WANG Ran  CHENG Jianjun  LI Qi  WANG Peng
Institution:LI Xiang,WANG Ran,CHENG Jianjun,LI Qi,WANG Peng(Food College,Northeast Agricultural University,Harbin 150030,China)
Abstract:L. plantarum was isolated from fermented waxy corn. The optimal formula of medium was studied in order to increase the total colonies of L. plantarum. Multiplication culture medium and the effects of different nutrient components on the grown of L. plantarum were investigated and optimized by single and orthogonal de-signs. The results showed that sucrose, yeast extract and KH2PO4 were the optimum factors. The total colonies of L. plantarum were 3.0×108 cfu·mL-1 in the optimized medium which was composed of 2% sucrose, 2.5% yeast extract, 0.2% KH2PO4.
Keywords:L  plantarum  culture medium  optimization  
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