| 番茄Cf-5基因的SNP分子标记开发 |
| |
| 引用本文： | 张育垚,李景富,谢立波,张贺,康立功,姜景彬,许向阳.番茄Cf-5基因的SNP分子标记开发[J].东北农业大学学报,2012,43(4):93-98. |
| |
| 作者姓名： | 张育垚 李景富 谢立波 张贺 康立功 姜景彬 许向阳 |
| |
| 作者单位： | 东北农业大学园艺学院,哈尔滨,150030 |
| |
| 基金项目： | 现代农业产业技术体系专项资金项目，国家科技支撑计划项目，东北农业大学创新团队项目，黑龙江省高等学校创新团队项目，东北农业大学博士启动基金，哈尔滨市科技创新人才研究专项资金项目 |
| |
| 摘 要： | 根据已知的叶霉病抗性基因Cf-5基因序列,设计了3对嵌套引物。以4份抗病材料和4份非抗病材料DNA为模板,扩增番茄Cf-5基因并比较了抗感材料Cf-5基因的序列,在580、2 879 bp位点分别有T与C、G与T的碱基替换,设计了等位基因特异引物,获得了稳定的等位基因PCR产物,成功地开发了抗叶霉病基因Cf-5的SNP标记,为培育抗叶霉病番茄品种奠定了基础。
|
| 关 键 词： | 番茄 Cf-5基因 SNP标记 |
Development of SNPs for leaf mould resistance gene Cf-5 in tomato |
| |
| ZHANG Yuyao
,
LI Jingfu
,
XIE Libo
,
ZHANG He
,
KANG Ligong
,
JIANG Jingbin
,
XU Xiangyang.Development of SNPs for leaf mould resistance gene Cf-5 in tomato[J].Journal of Northeast Agricultural University,2012,43(4):93-98. |
| |
| Authors: | ZHANG Yuyao LI Jingfu XIE Libo ZHANG He KANG Ligong JIANG Jingbin XU Xiangyang |
| |
| Institution: | (College of Horticulture,Northeast Agricultural University,Harbin 150030,China) |
| |
| Abstract: | Based on leaf mould resistant gene Cf-5 sequence,three nested primer pairs were designed for amplifying Cf-5 gene from genomic DNA of four resistant and four susceptible tomato accessions.Comparison of DNA sequences from resistant and susceptible accessions revealed two base substitutions(T to C and G to T at 580 and 2 879 bp,respectively).The specific primers were designed based on the SNP result and obtained a stable SNP marker for Cf-5 gene screening.It is a potential Cf-5 gene marker in tomato breeding. |
| |
| Keywords: | tomato Cf-5 gene SNP marker |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
