三种复方中药对鲫鱼肝脏抗氧化功能的影响

采用三种复方中药(编号分别为AB、CD和AH)分别饲喂1龄鲫鱼28 d,同时设置空白对照组,以探讨所用方剂对鲫鱼肝脏中抗氧化功能的影响。试验期间水温20℃,每天按照体重的2.0%投喂制备的饲料。采集鲫鱼肝脏,检测超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)、总抗氧化能力(T-AOC)四个代表性抗氧化指标。结果表明,三种复方中药组SOD活性均显著高于对照组。MDA含量均低于对照组,其中AB、CD组与对照组差异显著(P<0.05)。GSH-PX活性均高于对照组,其中AH、CD组与对照组差异显著(P<0.05)。三种复方中药均能提高T-AOC,其中AB、CD组提高效果显著(P<0.05)。所选的三种复方中药均对鲫鱼肝脏抗氧化能力有促进作用,其中CD组方效果最好。

Effect of three kinds of Chinese herbal compounds on antioxidation capability of liver in Crucian carp

In this study,the antioxidation capacity of liver in Crucian carp(one-year old) was investigated after feeding with three kinds of Chinese herbal compounds(they were numbered AB,CD and AH)continuously for 28 days.The control group was set at the same time.Two percents of fish body weight were fed at 20℃ every day during the experiment.Then the livers were sampled to detect the four anti-oxidative indexes including SOD,MDA,GSH-PX and T-AOC.The results showed that testing groups exhibited a significantly higher level of SOD activity while a lower level of MDA compared to control group.And there was a significant difference between the AB,CD groups and the control group(P<0.05).The GSH-PX activities were higher in the three testing groups compared to the control group,with a significant difference(P<0.05) between the AH,CD groups and the control group.The three kinds of Chinese herbal compounds could increase the total antioxidant capacity(T-AOC) obviously,especially in AB and CD groups.Based on the study results mentioned above,these three formula compounds could increase the liver anti-oxidative capacity in the Crucian carp,and CD group had the best effect.