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香水百合组织培养的试验研究
引用本文:阮少宁,杨华,梁一池,刘荣忠.香水百合组织培养的试验研究[J].福建林学院学报,2001,21(2):142-145.
作者姓名:阮少宁  杨华  梁一池  刘荣忠
作者单位:1. 福建林学院,
2. 漳州林业组培中心,
基金项目:福建省自然科学基金资助项目! (B9910 0 2 0 )
摘    要:通过筛选获得最佳的香水百合组培外植体 ,接种于 1 / 2 MS+ 2 ,4- D3 mg/L(单位下同 ) + 6 - BA1+ GA0 .1的诱导培养基上 ,诱导形成不定芽 ,且不定芽迅速形成小鳞茎 ,分化率达 93 .3 3 % .将不定芽接种到 1 / 2 MS+ 6 - BA0 .5的增殖培养基上 ,1个月左右小鳞茎增殖率可达到 1∶ 6 .80 .切取单个小鳞茎 ,考查不同基本培养基、蔗糖、生长素和活性碳浓度对小鳞茎复壮的影响 ,结果在培养基 3 / 4MS+ 6 - BA0 .6+ NAA0 .1+ GA0 .2 中生长的小鳞茎复壮效果最好 ,2 5d左右即可转入1 / 2 MS+ KT0 .3 + NAA0 .5的生根培养基中进行生根 .形成完整的百合小苗移栽至苗圃中 ,可打破休眠 ,使开花期提前

关 键 词:百合  组织培养  外植体
文章编号:1001-389X(2001)02-0142-04
修稿时间:2000年6月29日

Study on Tissue Culture of Lily
RUAN Shao-ning,YANG Hua,LIANG Li-chi,LIU Long-zhong.Study on Tissue Culture of Lily[J].Journal of Fujian College of Forestry,2001,21(2):142-145.
Authors:RUAN Shao-ning  YANG Hua  LIANG Li-chi  LIU Long-zhong
Abstract:While choosing the best explants of Lilium and inoculating it on inducing medium of 1/2MS+2,4-D3 mg/L+6-BA1+GA0.1, explants grow into adventitious bu ds and become little bulbs, whose dividing rate reaches 93.33%. After one month, advedentitions bud′s propagation rate reaches 1∶6.80 on propagation medium 1/2MS+6-BA0.5.Studying the effect of different based media, con centration of sugar, auxins and active carbon on regaining strong of the alone b ulb, the results show that the alone bulb can regain strong on medium 3/4MS+6-B A0.6+NAA0.1+GA0.2.Around 25 days,the aline buld can be in duced growing roots using 1/2 MS+KT0.3+NAA0.5,growing roots medium is good.The bulbs can grow many muscular roots a month later, and can become complete little seedlings. Transplanting these little se edlings can break dormancy and advance anthesis.
Keywords:Lily  tissue culture  explant
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