| 光皮桦RAPD分析体系优化设计方案比较 |
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| 引用本文： | 谢一青,黄儒珠,李志真,杨宗武,陈杰.光皮桦RAPD分析体系优化设计方案比较[J].福建林学院学报,2006,26(1):73-77. |
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| 作者姓名： | 谢一青 黄儒珠 李志真 杨宗武 陈杰 |
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| 作者单位： | 1. 福建师范大学生物工程学院,福建,福州350007
2. 福建省林业科学研究院,福建,福州,350012 |
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| 基金项目： | 福建省林木种苗科技攻关项目 |
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| 摘 要： | 采用单因素设计和均匀设计对光皮桦的RAPD反应体系进行了优化,并对2种设计方案优化出的最佳反应体系的可靠性和稳定性进行比较.结果表明:2种设计均可用于光皮桦RAPD体系的优化,但单因素设计受多因素间交互作用的影响使反应体系达不到最优;而均匀设计能避免盲目性,并快速得到条带清晰、稳定性好的适合光皮桦的最优RAPD体系.
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| 关 键 词： | 光皮桦 RAPD 优化 均匀设计 单因素设计 |
| 文章编号： | 1001-389X（2006）01-0073-05 |
| 收稿时间： | 2005-10-26 |
| 修稿时间： | 2005-12-19 |
Comparison between single factor design and uniform design to optimize the reaction system for RAPD of Betula luminifera |
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| XIE Yi-qing,HUANG Ru-zhu,LI Zhi-zhen,YANG Zong-wu,CHEN Jie.Comparison between single factor design and uniform design to optimize the reaction system for RAPD of Betula luminifera[J].Journal of Fujian College of Forestry,2006,26(1):73-77. |
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| Authors: | XIE Yi-qing HUANG Ru-zhu LI Zhi-zhen YANG Zong-wu CHEN Jie |
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| Institution: | 1. College of the Bioengineering, Fujian Normal University, Fuzhou 350007, China; 2. Fujian Academy of Forestry, Fuzhou 350012, China |
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| Abstract: | Single factor design and uniform design were used to obtain optimum scheme on reaction system for random amplified polymorphic DNA(RAPD) of Betula luminifera.And the optimum reaction systems of the two designs were compared.Results showed that both single factor design and uniform design could be used to optimize the reaction system for RAPD of B.luminifera,and single factor design caused by the effect of interaction on multifactor and could not obtain the optimum reaction system.But uniform design could avoid blindness and quickly obtained the optimum reaction system for RAPD of B.luminifera with clear bands and good stability. |
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| Keywords: | Betula luminifera random amplified polymorphic DNA optimization uniform design single factor design |
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