| 木兰科濒危植物大果木莲遗传多样性的ISSR分析 |
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| 引用本文： | 陈少瑜,韩燕,吴涛,付玉嫔,司马永康,郝佳波.木兰科濒危植物大果木莲遗传多样性的ISSR分析[J].福建林学院学报,2010,30(1). |
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| 作者姓名： | 陈少瑜 韩燕 吴涛 付玉嫔 司马永康 郝佳波 |
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| 作者单位： | 1. 云南省林业科学院,云南省森林植物培育与开发利用重点实验室,云南,昆明,650204
2. 西南林学院资源学院,云南,昆明,650224 |
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| 基金项目： | 云南省自然科学基金资助项目(2006C0066M) |
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| 摘 要： | 采用ISSR分子标记技术对云南省3个大果木莲天然居群进行遗传多样性分析。结果表明,在物种水平上,大果木莲的多态位点百分率(PPB)为70.71%;有效等位基因数(Ne)为1.4121;Nei′s基因多样性指数(H)为0.2433;Shannon信息指数(I)为0.3651。在居群水平上,其PPB为44.1%;Ne为1.2704;H为0.1573;I为0.2343。通过Nei′s遗传多样性分析得到居群间的遗传分化系数(Gst)为0.3595,由遗传一致度进行了3个居群的UPGMA聚类。
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| 关 键 词： | 大果木莲 濒危植物 ISSR分子标记 遗传多样性 |
Genetic diversity of endangered species Manglietia grandis detected by inter-simple sequence repeat markers |
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| CHEN Shao-yu,HAN Yan,WU Tao,FU Yu-pin,SIMA Yong-kang,HAO Jia-bo.Genetic diversity of endangered species Manglietia grandis detected by inter-simple sequence repeat markers[J].Journal of Fujian College of Forestry,2010,30(1). |
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| Authors: | CHEN Shao-yu HAN Yan WU Tao FU Yu-pin SIMA Yong-kang HAO Jia-bo |
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| Institution: | 1.The Key Laboratory of Forest Plant Cultivation and Utilization;Yunnan Academy of Forestry;Kunming;Yunnan 650204;China;2.Resource Department of Southwest Forestry College;Yunnan 650224;China |
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| Abstract: | Inter-simple sequence repeat (ISSR) technique was used to detect 3 natural populations of Manglietia grandis distributed in Yunnan.The results showed that at species level,the value of the average percentage of polymorphic bands (PPB) was 70.71%,effective number of alleles per locus (Ne) was 1.412 1,Nei's gene diversity index (H) was 0.243 3 and Shannon's information index (I) was 0.365 1.While at population level,the values of PPB,Ne,H and I were 44.1%,1.270 4,0.157 3 and 0.234 3 respectively.The coefficie... |
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| Keywords: | Manglietia grandis endangered species ISSR markers genetic diversity |
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