| 白僵菌和绿僵菌蛋白对松墨天牛致病性的研究 |
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| 引用本文： | 李建庆,张永安,梅增霞,石东里.白僵菌和绿僵菌蛋白对松墨天牛致病性的研究[J].福建林学院学报,2006,26(2):165-168. |
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| 作者姓名： | 李建庆 张永安 梅增霞 石东里 |
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| 作者单位： | 1. 滨州学院生命科学系,山东,滨州,256600
2. 中国林业科学研究院森林生态环境与保护研究所,北京,100091 |
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| 基金项目： | 国家重点基础研究发展规划“973”项目(G19990160) |
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| 摘 要： | 从4株白僵菌和1株绿僵菌中提取了10种全蛋白提取液,以林业害虫松墨天牛幼虫为试虫,筛选出菌株Bb36的全蛋白提取液Bb36W-Z和Bb36N-Z,再从中分离出12种单一蛋白提取液,生测发现虫生真菌蛋白Bb36W-D对松墨天牛幼虫具有较强的注射毒性,经其处理后的试虫酯酶谱发生明显变化,致使松墨天牛幼虫内部组织发生病变.
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| 关 键 词： | 松墨天牛 致病蛋白 球孢白僵菌 金龟子绿僵菌 |
| 文章编号： | 1001-389X（2006）02-0165-04 |
| 收稿时间： | 2005-11-29 |
| 修稿时间： | 2006-02-13 |
Pathogenicity of entomogenous fungi proteins to Monochamus alternatus |
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| LI Jian-qing,ZHANG Yong-an,MEI Zeng-xia,SHI Dong-li.Pathogenicity of entomogenous fungi proteins to Monochamus alternatus[J].Journal of Fujian College of Forestry,2006,26(2):165-168. |
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| Authors: | LI Jian-qing ZHANG Yong-an MEI Zeng-xia SHI Dong-li |
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| Institution: | 1. Department of Life Sciences, Binzhou University, Binzhou 256603, China; 2. The Research Institute of Forest Ecology, Environment and Protection, CAF, Beijing 100091, China |
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| Abstract: | Ten total protein extractions were purified from four strains of Beauveria bassiana and one Metarhizium anisopliae.Total protein extraction Bb36W-Z and Bb36N-Z from strain Bb36 were screened by bioassay which objection was Monochamus alternatus larvae,a serious forest pest.Twelve proteins were injected M.alternatus larvae after isolated from Bb36W-Z and Bb36N-Z.It was discovered that protein Bb36W-D was higher pathogenicity than others,and caused M.alternatus larvae pathological changes(because isozyme patterns)of the treatment was different from the control. |
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| Keywords: | Monochamus alternatus pathogenic protein Beauveria bassiana Metarhizium anisopliae |
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