| 雷竹花粉萌发率及贮藏方法研究 |
| |
| 引用本文： | 刘倩倩,徐一帆,林新春,方伟.雷竹花粉萌发率及贮藏方法研究[J].福建林学院学报,2012,32(2):146-150. |
| |
| 作者姓名： | 刘倩倩 徐一帆 林新春 方伟 |
| |
| 作者单位： | 浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安,311300 |
| |
| 基金项目： | 国家自然科学基金资助项目，浙江省优先主题重点农业项目 |
| |
| 摘 要： | 为更好地开展竹类植物的杂交育种工作,对雷竹花粉的萌发率及贮藏方法进行了研究。结果表明,利用离体花粉萌发法测定的雷竹花粉萌发率远低于氯化三苯四氮唑(TTC)染色法测定的花粉生活力;添加50 mg.L-1硼酸有利于雷竹花粉萌发;不同发育阶段的花粉萌发率有明显差异,以花药完全露出未开裂的花粉萌发率为最高;花粉采集时间以15:30优于9:30;雷竹花粉不耐贮藏,4℃湿藏有利于保持花粉生活力。
|
| 关 键 词： | 雷竹 花粉生活力 花粉萌发率 贮藏 |
Pollen germination and storage of Phyllostachys violascens |
| |
| LIU Qian-qian
,
XU Yi-fan
,
LIN Xin-chun
,
FANG Wei.Pollen germination and storage of Phyllostachys violascens[J].Journal of Fujian College of Forestry,2012,32(2):146-150. |
| |
| Authors: | LIU Qian-qian XU Yi-fan LIN Xin-chun FANG Wei |
| |
| Institution: | (Nurturing Station for the State Key Laboratory of Subtropical Silviculture,Zhejiang Agriculture and Forestry University,Lin′an,Zhejiang 311300,China) |
| |
| Abstract: | Pollen germination and storage of Phyllostachys violascens were studied for bamboo crossbreeding.The results were summarized as follows: the pollen germination rate measured by in vitro pollen germination was lower than the pollen viability rate detected by triphenyltetrazolium chloride triphenyltetrazolium chloride(TTC) staining technique;the pollen germination ability could be improved by supplementing with 50 mg · L-1 boric acid in the medium;the pollen germination rate of the anthers completely outcrop but not dehiscent was higher than those of other development stages;as for the pollen collection time,15:30 was better than 9:30;pollen viability of Ph.violascens would be lost quickly during storage,and certain humidity and low temperature at 4℃ could be beneficial to keep the pollen viability. |
| |
| Keywords: | Phyllostachys violascens pollen viability pollen germination rate storage |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
