杉木不同器官愈伤组织和再生芽的诱导效率

以杉木未成熟胚、种子发芽的下胚轴、子叶以及组培苗茎段为外植体,研究基本培养基、不同激素组合及浓度对杉木愈伤组织的诱导及再生芽发生的影响。结果表明:不同激素组合及浓度对不同外植体的愈伤组织诱导率有显著影响,对未成熟胚愈伤组织诱导最佳的培养基为MS+2,4-D 2 mg·L~(-1)+6-BA 1 mg·L~(-1)+KT 1 mg·L~(-1),诱导率为92.7%;对子叶、下胚轴愈伤组织诱导最佳的培养基为MS+6-BA 2 mg·L~(-1)+NAA 2 mg·L~(-1)+TDZ 0.01 mg·L~(-1),诱导率分别为97.4%和95.2%;对组培苗茎段愈伤组织诱导最佳的培养基为MS+2,4-D 1 mg·L~(-1)+NAA 2 mg·L~(-1)+TDZ 0.01 mg·L~(-1),诱导率为86.7%;MS+6-BA 0.5 mg·L~(-1)+KT 1.5 mg·L~(-1)是愈伤组织诱导再生芽的最佳培养基,诱导率为80%。质地疏松的愈伤组织其再生芽率最高(65%),单个质地均匀的愈伤组织其再生芽数可达6个;基本培养基对愈伤组织质地形态有较大影响,1/2MS培养基诱导出愈伤组织质地较疏松,MS培养基诱导出的愈伤组织质地均匀且表面可见颗粒状细胞团,DCR培养基诱导出的愈伤组织质地较密。

Efficiency of callus and regeneration bud induction from different explants of Cunninghamia lanceolata

For the purpose of establishing callus induction and bud regenerate system for Cunninghamia lanceolata (Lamb.) Hook.,and proving an effective receptor procedure for researching into the genetic engineering of somatic hybridization and genetic transformation.Using immature embryo,hypocotyls and cotyledons of seedlings and stems of sterile seedlings as explants,the effect of the following factors including basal medium,different types of hormone concentrations and combinations on callus induction and regeneration buds differentiation were investigated respectively.The results indicated that different hormone combinations on callus induction of different explants have a significant influence.The optimal medium for immature embryo induction callus was MS+2,4-D 2 mg·L-1 +6-BA 1 mg·L-1 +KT 1 mg·L-1,the induction rate was 92.7％;the optimal medium for cotyledons and hypocotyls induction callus was MS+6-BA 2 mg·L-1+NAA 2 mg·L-1+TDZ 0.01 mg·L-1,the induction rates were 97.4％ and 95.2％ respectively;the optimal medium for stems from sterile seedlings induction callus was MS+2,4-D 1 mg·L-1+NAA 2 mg·L-1 +TDZ 0.01 mg·L-1,the induction rate was 86.7％;the optimal medium for callus induction regeneration bud was MS+6-BA 0.5 mg· L-1 +KT 1.5 mg· L-1,the induction rate was 80％.The callus with loose texture has the highest regeneration rate (65％),but for a single callus with homogeneous texture,its maximum number of regenerated buds was 6.The basic medium has great influence on the texture of callus that the callus induced by 1/2MS basal medium showed loose texture,homogeneous surface with visible granular cell for MS basal medium,and dense callus for DCR basal medium.