仿刺参不同组织液的抗菌活性

采用平板菌落计数法测定仿刺参Apostichopus japonicus体腔液、体腔液上清液、体腔细胞悬液和体壁内皮组织匀浆液对哈氏弧菌Vibrio harveyi的抑制作用,并对健康仿刺参与感染后仿刺参的体壁内皮组织匀浆液的抑菌作用进行了比较;采用乙醇沉淀、离心等方法从仿刺参体壁内皮组织中粗提得到一种水溶性成分,测定了该成分对哈氏弧菌、白色葡萄球菌Staphylococcus albus和迟钝爱德华氏菌Edwardsiella tarda的抑菌活性,并初步研究了该成分的基本性质。结果表明：仿刺参体壁内皮组织匀浆液对哈氏弧菌的抑制效果显著,体腔液、体腔液上清液、体腔细胞悬液对哈氏弧菌的抑制效果不显著;感染状态下,仿刺参体壁内皮组织匀浆液的抑菌作用与健康状态相比有所降低。提取的水溶性成分对哈氏弧菌、白色葡萄球菌和迟钝爱德华氏菌均有明显的抑制作用;该成分的茚三酮反应呈阳性,其中蛋白质的质量分数为17.48%;该成分经SDS聚丙烯酰胺凝胶电泳分离得到5个蛋白条带,相对分子质量大约为55 300、46 100、34 700、19 500和14 500,初步认为其含有肽类抗菌活性物质。

Antibacterial activity of different tissue components in sea cucumber Apostichopus japonicus

The inhibitions of strain H06091（Vibrio harveyi） of coelomic fluid（containing coelomocyte）,suspension of coelomocyte（without coelomocyte）,and suspension of coelomocyte and homogenate of endothelial tissue were detected in healthy and challenged sea cucumber Apostichopus japonicus by colony counting methods.The inhibition to V.harveyi,Staphylococcus albus and Edwardsiella tarda and basic characteristics were studied in water-solubility component from homogenate of endothelial tissue of the sea cucumber by ethanol precipitation and centrifuging method.The results showed that the endothelial homogenate had significant inhibition of V.harveyi,but the coelomic fluid containing coelomocytes,suspension of coelomocyte（without coelomocyte）and suspension of coelomocytes had no inhibition.The inhibition of the endothelial homogenate to bacteria was found to be reduced in the infected sea cucumber.The water-solubility component showed inhibition of V.harveyi,S.albus and E.tarda.The water-solubility component was found positive ninhydrin reaction and had protein content of 17.48% with five bands,molecular weight of about 55 300,46 100,34 700,19 500 and 14 500 by SDS-PAGE.The water-solubility component from the endothelial homogenate of the sea cucumber was showed to contain antimicrobial peptides.