| 裙带菜凝集素对日本对虾非特异性免疫因子的影响 |
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| 引用本文： | 李丹彤,张静,陈国栋,邢殿楼,余根英,宁凯,吴振海.裙带菜凝集素对日本对虾非特异性免疫因子的影响[J].大连水产学院学报,2009,24(3):274-278. |
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| 作者姓名： | 李丹彤 张静 陈国栋 邢殿楼 余根英 宁凯 吴振海 |
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| 作者单位： | 大连水产学院,农业部海洋水产增养殖学重点开放实验室,辽宁,大连,116023 |
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| 基金项目： | 大连市科学技术基金资助项目 |
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| 摘 要： | 为了研究裙带菜凝集素对日本对虾非特异性免疫因子的影响,给日本对虾Penaeus japonicus注射了2.3 mg/尾裙带菜凝集素,并于注射后第24、48、72、96、120 h抽血取样,测定对虾体内8种非特异性免疫指标。结果表明：试验组日本对虾血清中的凝集活性、酚氧化酶活性、超氧化物歧化酶活性、碱性磷酸酶活性、溶菌酶活性在24-120 h时均高于对照组。其中凝集活性、酚氧化酶活性在24 h时最大,分别是对照组的8倍、64倍;超氧化物歧化酶活性在48 h时最大,是对照组的2.6倍;碱性磷酸酶活性、溶菌酶活性在72 h时最大,分别是对照组的2.6倍、4.2倍;抗菌活性在24、72-120 h时均高于对照组,并且在24 h时最大,是对照组的1.3倍;吞噬率和吞噬指数在24-72 h、120 h时均高于对照组,并且在72 h时最大,分别是对照组的1.2倍、1.4倍。研究表明,注射裙带菜凝集素后可以明显提高日本对虾的非特异性免疫力。
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| 关 键 词： | 裙带菜 凝集素 日本对虾 非特异性免疫因子 |
Effect of lectin from sea mustard Undaria pinnatifida on non-specific immunity in kuruma shrimp Penaeus japonicus |
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| LI Dan-tong,ZHANG Jing,CHEN Guo-dong,XING Dian-lou,YU Geng-ying,NING Kai,WU Zhen-hai.Effect of lectin from sea mustard Undaria pinnatifida on non-specific immunity in kuruma shrimp Penaeus japonicus[J].Journal of Dalian Fisheries University,2009,24(3):274-278. |
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| Authors: | LI Dan-tong ZHANG Jing CHEN Guo-dong XING Dian-lou YU Geng-ying NING Kai WU Zhen-hai |
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| Institution: | LI Dan-tong,ZHANG Jing,CHEN Guo-dong,XING Dian-lou,YU Geng-ying,NING Kai,WU Zhen-hai(Key Laboratory of Mariculture,Agriculture Ministry,PRC,Dalian Fisheries Univ.,Dalian 116023,China) |
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| Abstract: | Effects of lectin from sea mustard Undaria pinnatifida on non-specific immunity in kuruma shrimp Penaeus japonicus weighing 6-7 g were studied by injection of lectin at a dose of 2.3 mg per individual 24,48,72,96,and 120 h after injection.The results showed that the shrimp with injection of lectin had higher activities of hemagglutination,phenoloxidase(PO),and superoxide dismutase(SOD),alkaline phosphatase(ALP) and lysozyme(LYS) in the serum than those in the control group from 24 to 120 h after injection.T... |
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| Keywords: | algal lectin Penaeus japonicus non-specific immunity Undaria pinnatifida |
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