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皱纹盘鲍血细胞分类及活性氧产生机理的研究
引用本文:张剑诚,张峰,王吉桥.皱纹盘鲍血细胞分类及活性氧产生机理的研究[J].大连水产学院学报,2004,19(3):182-188.
作者姓名:张剑诚  张峰  王吉桥
作者单位:大连水产学院,农业部海洋水产增养殖学与生物技术重点开放实验室,辽宁,大连,116023
基金项目:国家"九七三"资助项目(G1999012005)
摘    要:研究了皱纹盘鲍Haliotis discus hannai Ino血细胞的组成、数量、吞噬作用及活性氧产生机理。皱纹盘鲍血细胞分为透明细胞和颗粒细胞两大类。透明细胞无颗粒,具嗜碱性细胞质,约占循环血细胞总量的60%;颗粒细胞含嗜酸性颗粒。这两种血细胞都有吞噬作用,尤其是颗粒细胞,经酵母细胞刺激后,血细胞释放活性氧,还原氮蓝四唑(NBT)形成甲欣沉淀,有化学发光现象。长期饥饿的皱纹盘鲍,血细胞活性氧量低于正常水平。

关 键 词:皱纹盘鲍  免疫  血细胞  活性氧
文章编号:1000-9957(2004)03-0182-07

Classification of hemocytes and mechanism of production of reactive oxygen species in abalone Haliotis discus hannai Ino
ZHANG Jian-cheng,ZHANG Feng,WANG Ji-qiao C,Dalian Fisheries Univ.,Dalian ,China.Classification of hemocytes and mechanism of production of reactive oxygen species in abalone Haliotis discus hannai Ino[J].Journal of Dalian Fisheries University,2004,19(3):182-188.
Authors:ZHANG Jian-cheng  ZHANG Feng  WANG Ji-qiao C  Dalian Fisheries Univ  Dalian  China
Institution:ZHANG Jian-cheng,ZHANG Feng,WANG Ji-qiao C,Dalian Fisheries Univ.,Dalian 116023,China)
Abstract:Morphology and cytochemistry of haemocytes in abalone, Haliotis discus hannai Ino, were described and their role in phagocytosis and encapsulation and mechanism of reactive oxygen species (ROS) production were studied. The hemocytes of abalone can be divided into two types: hyalinocytes(HY) and granulocytes (GR) based on the presence of granulates. There are granular particulates in HY with a basophilic cytoplasm, whereas GR contained eosinophilic granules. The HY comprised approximately 60% of the hemocytes in the abalone circulation. Both of the haemocytes, especially GR, were actively phagocytic. After stimulated with yeast, the blood cells were able to produce ROS which reduced NBT to formazan, accompanied by chemiluminescence (CL). There were less hemocytes and lower rate of the ROS production in starved abalones than those in the normal ones.
Keywords:Haliotis discus hannai Ino  immune system  hemocyte  reactive oxygen
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