迟钝爱德华氏菌黏附及侵袭特性的研究

将引发养殖大菱鲆Scophthalmus maximus出血性败血症的病原菌迟钝爱德华氏菌Edwardsiella tarda（L-49231）接种于体外培养细胞—人上皮角质层形成细胞（HaCaT—cell），观察其对细胞的黏附性和侵袭性，研究了不同作用时间下以及用不同因素处理菌体后，细菌对HaCaT细胞黏附性及侵袭性的影响。结果表明：L-49231菌株能黏附HaCaT细胞，2h后即可侵入细胞；随着作用时间的延长，细菌黏附细胞与侵入细胞的数量增加。分别用甲醛、戊二醛、盐酸、胰蛋白酶处理L-49231菌体，细菌的黏附力及侵袭力均有所下降；用蔗糖、甘露糖处理L-49231菌体时，细菌的黏附力变化不大；用葡萄糖处理时，能够促进细菌对细胞的黏附作用。用甘露糖和葡萄糖处理菌体时，能促进细菌对细胞的侵袭；用蔗糖处理菌体时，能部分抑制细菌对细胞的侵袭作用。用抗体处理L-49231菌体后，细菌的黏附力及侵袭力均明显下降。说明L-49231菌株对HaCaT细胞具明显的侵袭性，其主要黏附因子为胞外蛋白质成分，无确切的糖受体。

Invasion and virulence of pathogenic bacterium Edwardsiella tarda

A strain isolated from the diseased turbot Scophthalmus maximus was proved to be the pathogenic bacteria by an artificial infection test.The strain was identified as Edwardsiella tarda numbered as L-49231 strain.The adhesion and invasion of strain L-49231 to HaCaT cell was observed by being inoculated in 24-well cell culture plates.The adhesion and invasion of the L-49231 strain contacted to HaCaT cell during different period and transacted with different factors were studied.The results showed that the L-4...