五味子提取物对用t-BHP损伤的异育银鲫原代肝细胞的影响

以叔丁基氢过氧化物（t-BHP）诱导异育银鲫Carassius auratus gibel原代培养肝细胞损伤模型，采用不同的给药顺序，通过检测肝细胞培养上清液中谷丙转氨酶（ALT／GPT）、微量丙二醛（MDA）、谷光甘肽过氧化物酶（GSH—PX）和超氧化物歧化酶（SOD）的含量以及肝细胞的增殖活性，研究了五味子提取物对急性肝细胞损伤的保护作用。结果表明：以浓度为1mmoL／L的t—BHP作用肝细胞2h诱导肝损伤模型，加入五味子提取物后能通过提高上清液中GSH—PX和SOD酶活力以及抑制脂质过氧化产物MDA的生成来减轻t—BHP对肝细胞的损伤，减少GPT的释放，使GPT活力水平的升高受到明显抑制，显著提高了肝细胞的存活率（P〈0．05或P〈0．01）；预防治疗组（DT）中五味子提取物对损伤肝细胞的保护效果明显要优于预防组（D）和治疗组（T）。说明五味子提取物对由t—BHP造成的肝细胞急性损伤具有一定的保护作用，该保护作用可能与其抗氧化、清除自由基的能力有关；中药与损伤剂的给予顺序会影响五味子提取物对肝细胞的保护作用。

Protective effects of schisandrins extracts on injuried primary cultured hepatocytes induced by t-BHP in crucian carp Carassius auratus gibel

The activities of inhibiting alanine minotransferase （ GPT）, malondisldehyde （ MDA）, superoxide dismutase （SOD）, and glutathione peroxidas （GSH-PX） in cell culture supernatants and the survival rate of the injuried hepatocytes in vitro induced by t-BHP and then exposed to various concentrations of Schisandrins extracts （0. 1, 0. 2 and 0.4 mg/mL） before （defense）, after （treatment） and both before and after （defense-treatment） the culture were determined in the crucian carp Carassius auratus gibel to study on the protective and antioxidant effects of the Schisandrins extract. The model of acute hepatopancreatic damage was found to be established by exposure to 1 mmoL/L t-BHP for 2 h. The extracts were found to reduce the injury of hepatopancrease by increasing the activity of glutathione peroxidas （GSH-PX） and superoxide dismutase （SOD）, restraining the lipid peroxidation Production-malondisldehyde （MDA）, inhibiting alanine minotransferase （GPT）, to release and to remarkably improve the survival of the hepatocytes （ P〈0.05, or P〈0. Ol ） in the supernatant of cultured hepatocytes injured by t-BHP induction. It is concluded that the Schisandrins extracts possess direct protective effect on primary hepatocyte injury induced by t-BHP, which is primarily involved in the scavenging oxygen free radicals and anti-oxida- tive activity of CMPS. The biochemical indicators showed that the protection of the extracts were significantly better in the defense-treatment group （DT） than that in the defense group （D） and treatment group （T）