| 哲罗鲑雌性蛋白的研究 |
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| 引用本文： | 王金燕,张颖,王凤,霍堂斌,尹家胜.哲罗鲑雌性蛋白的研究[J].大连水产学院学报,2010,25(5):422-427. |
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| 作者姓名： | 王金燕 张颖 王凤 霍堂斌 尹家胜 |
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| 作者单位： | 1. 中国水产科学研究院,黑龙江水产研究所,哈尔滨,150070;上海海洋大学,水产与生命学院,上海,201306
2. 中国水产科学研究院,黑龙江水产研究所,哈尔滨,150070 |
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| 基金项目： | 国家自然科学基金资助项目(2006BAD03B08) |
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| 摘 要： | 采用柱层析、蛋白质免疫印迹和电泳等方法,对哲罗鲑Hucho taimen的雌性蛋白进行了系统研究。结果表明：哲罗鲑血清和卵黄中均存在雌性蛋白,其雌性蛋白的性质相同,均为糖脂磷蛋白;与血清雌性特异蛋白一样,卵黄雌性特异蛋白的相对分子质量也为460 000,由相对分子质量分别为137 800、84 200和10 800的3个亚基组成,等电点为5.60;卵黄雌性蛋白的兔抗血清均与哲罗鲑雌性血清发生免疫反应,不与雄性血清发生免疫反应,表明这两种蛋白均具有雌性特异性;两种蛋白的抗血清可以相互检测,表明哲罗鲑雌鱼血清中雌性蛋白与卵黄中的雌性蛋白在免疫原性上和结构上具有较高的同源性。
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| 关 键 词： | 哲罗鲑 血清 卵黄 雌性蛋白 |
Female-related protein in taimen Hucho taimen |
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| WANG Jin-yan,ZHANG Ying,WANG Feng,HUO Tang-bin,YIN Jia-sheng.Female-related protein in taimen Hucho taimen[J].Journal of Dalian Fisheries University,2010,25(5):422-427. |
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| Authors: | WANG Jin-yan ZHANG Ying WANG Feng HUO Tang-bin YIN Jia-sheng |
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| Institution: | WANG Jin-yan1,2,ZHANG Ying1,WANG Feng1,HUO Tang-bin1,YIN Jia-sheng1 (1.Heilongjiang Fishery Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China,2.College of Fisheries , Aquatic Life Science,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | Female-related protein was purified from serum and egg yolk in broodstock taimen Hucho taimen by chromatography and electrophoresis,and the chemical properties were analyzed.The results showed that the FYSP protein was observed in the second peak of the crude extract in the eggs,with identical characters with female special serum protein existed in the female serum.SDS-PAGE revealed that the two kinds of FYSP with molecular weights of 137 800,84 200 and 10 800 appeared as three bands.These proteins were sta... |
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| Keywords: | Hucho taimen female-related protein serum egg |
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