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| 菲律宾蛤仔凝集素的分离纯化及抑菌活性研究 | |
| 引用本文： | 李伟,陈文,佟长青,孔亮,曲敏,谭成玉,金桥,李丹彤.菲律宾蛤仔凝集素的分离纯化及抑菌活性研究[J].大连水产学院学报,2012,27(4):333-337. |
| 作者姓名： | 李伟 陈文 佟长青 孔亮 曲敏 谭成玉 金桥 李丹彤 |
| 作者单位： | 1. 大连海洋大学食品科学与工程学院,辽宁大连,116023 2. 大连海洋大学食品科学与工程学院,辽宁大连116023;东北林业大学盐碱地生物资源环境研究中心,东北油田盐碱植被恢复与重建教育部重点实验室,黑龙江哈尔滨150040 3. 大连海洋大学海洋环境工程学院,辽宁大连,116023 4. 大连海洋大学水产与生命学院,辽宁大连,116023 |
| 基金项目： | 国家自然科学基金资助项目（31071612,21075012） |
| 摘 要： | 采用Cellulose DE52离子交换层析、Sephadex G- 100分子筛层析及TSK G3000PWXL凝胶色谱柱HPLC从菲律宾蛤仔Ruditapes philippinarum中分离纯化出具有N-乙酰半乳糖酰胺(N- acetyl- D- galactosamine,GaINAc)和猪胃黏蛋白(Mucin from porcine stomach,PSM)特异性的菲律宾蛤仔凝集素(简称为MCL-T).SDS-PAGE分析结果表明,MCL-T的相对分子量为148000,含有62000和36000的亚基.抑菌试验结果表明,MCL-T对金黄色葡萄球菌Staphylococcus aureus和枯草芽孢杆菌Bacillus subtilis有较高的抑菌活性.对MCL-T中Trp残基、-S-S-、-SH以及Tyr残基进行化学修饰后,MCL-T对金黄色葡萄球菌的抑菌活性丧失；对其-S-S-进行化学修饰后,MCL-T对枯草芽孢杆菌的抑菌活性影响不大,而对其-SH、Trp残基和Tyr残基进行化学修饰后,MCL-T对枯草芽孢杆菌的抑菌活性丧失.研究表明,MCL-T 肽链上的氨基酸残基状态与其抑菌活性有关. |
| 关 键 词： | 菲律宾蛤仔 凝集素 抗菌活性 化学修饰 |
Isolation,purification and antibacterial activity of lectin from Manila clam Ruditapes philippinarum |
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| LI Wei , CHEN Wen , TONG Chang-qing , KONG Liang , QU Min , TAN Cheng-yu , JIN Qiao , LI Dan-tong.Isolation,purification and antibacterial activity of lectin from Manila clam Ruditapes philippinarum[J].Journal of Dalian Fisheries University,2012,27(4):333-337. | |
| Authors: | LI Wei CHEN Wen TONG Chang-qing KONG Liang QU Min TAN Cheng-yu JIN Qiao LI Dan-tong |
| Institution: | 1.College of Food Science and Engineering,Dalian Ocean University,Dalian 116023,China;2.Alkali Soil Natural Environmental Science Center,Northeast Forestry University/Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field,Ministry of Education,Harbin 150040,China;3.College of Marine Science and Environment,Dalian Ocean University,Dalian 116023,China;4.College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China) |
| Abstract: | A N-acetyl-D-galactosamine（GalNAc）-/mucin from porcine stomach（PSM）-specific lectin（MCL-T） was purified from Manila clam Ruditapes philippinarum using a combination of ion exchange on Cellulose DE52 and gel filtration on Sephadex G-100 and HPLC on TSK-GEL G3000PWXL.Molecular weight of the MCL-T was determined by SDS-PAGE to be 148 000,containing two kinds of subunits,62 000 and 36 000.The MCL-T was found to show the antibacterial activities against Staphylococcus aureus and Bacillus subtilis.The antibacterial activities to Staphylococcus aureus was found to be disappeared in the MCL-T in which tryptophan residues,-S-S-,-SH and tyrosine residues were chemically modified.The chemical modification of-S-S-in the MCL-T led to less effects on the antibacterial activities against Bacillus subtilis,but the chemical modification of its-SH,tryptophan residues and tyrosine residues resulted in losing antibacterial activities against Bacillus subtilis.The findings indicate that the patterns of the MCL-T amino acid residues is involved in the antibacterial activities. |
| Keywords: | Ruditapes philippinarum lectin antibacterial activity chemical modification |
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