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仿刺参肠多糖提取物对小鼠实体瘤的抑制作用
引用本文:王斌,袁甜,张译文,王志远,秦英玲,薛仙子,常亚青.仿刺参肠多糖提取物对小鼠实体瘤的抑制作用[J].大连水产学院学报,2012,27(3):195-199.
作者姓名:王斌  袁甜  张译文  王志远  秦英玲  薛仙子  常亚青
作者单位:1. 大连海洋大学 农业部北方海水增养殖重点实验室,辽宁 大连,116023
2. 中南大学 湘雅医学院,湖南 长沙,410013
摘    要:采用碱解、酶解和醇沉淀法提取仿刺参Apostichopusjaponicus肠组织中的粗多糖(简称肠多糖),通过构建小鼠I-122肝癌实体瘤模型,采用腹腔给药途径,研究了不同剂量的肠多糖200、100、50mg/(kg·d)]对小鼠实体瘤的抑制作用,并测定了小鼠的脾脏指数、胸腺指数以及血清中肿瘤坏死因子(TNF—α)和白细胞介索2(IL-2)的含量。结果表明:肠多糖对患H22肝癌小鼠的实体瘤具有显著的抑制作用,肠多糖高剂量组小鼠的瘤质量与肿瘤对照组差异极显著(P〈0.01),肠多糖中、低剂量组的瘤质量与肿瘤对照组差异显著(P〈0.05),肠多糖高、中、低剂量的抑瘤率分别为90.1%、85.2%和71.7%;各肠多糖组小鼠的脾脏指数与肿瘤对照组均无显著差异(P〉0.05),但随着肠多糖剂量的上升,小鼠的脾脏指数也上升;各肠多糖组小鼠的胸腺指数均高于肿瘤对照组,除肠多糖低剂量组外其余各组均与肿瘤对照组差异显著(P〈0.05);肠多糖高剂量组小鼠血清中的IL-2含量极显著高于3个对照组(P〈0.01),而肠多糖中、低剂量组血清中的IL-2含量与肿瘤对照组、给药健康对照组均无显著差异(P〉0.05);肠多糖高、中、低剂量组小鼠血清中的TNF—α含量与3个对照组均无显著差异(P〉0.05)。

关 键 词:仿刺参  肠多糖  H22肝癌荷瘤小鼠  抗肿瘤作用

The antitumor effect of intestinal polysaccharides in sea cucumber Apostichopus japonicus
WANG Bin , YUAN Tian , ZHANG Yi-wen , WANG Zhi-yuan , QIN Ying-ling , XUE Xian-zi , CHANG Ya-qing.The antitumor effect of intestinal polysaccharides in sea cucumber Apostichopus japonicus[J].Journal of Dalian Fisheries University,2012,27(3):195-199.
Authors:WANG Bin  YUAN Tian  ZHANG Yi-wen  WANG Zhi-yuan  QIN Ying-ling  XUE Xian-zi  CHANG Ya-qing
Institution:1. Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China; 2. Xiangya Shool of Medicine Central South University, Changsha 410013 ,China )
Abstract:The polysaccharides were extracted from intestines of sea cucumber Apostichopus japonicus by alkaline hydrolysis and enzymatic hydrolysis, and H22 tumor bearing mouse were established. The inhibiting effect of intes- tinal polysaccharide to H22 tumor were studied by celiac injection of polysaccharides at different doses( 200,100 mg/( kg d) and 50 rag/( kg d) ). The tumor inhibiting rates, thymus index, spleen index, IL-2, and TNF-α were determined 15 days after intestinal polysaccharide injection. The sea cucumber injected by the intestinal poly- saccharides showed anti-tumor efficacy on H22 tumor bearing mice. There were significant higher tumor inhibition rates in the polysaccharide treatment groups than that in the control group ( high dose group P〈0. 01 ; middle, low dose groups P〈0. 05). The spleen index was found to be increased with dose increase in the polysaccharide treat- ment groups compared with the control group, without significant difference. There was higher thymus index in the polysaccharide treatment groups than that in the tumor control group (P〈0. 05). The maximal IL-2 content was observed in the high dose polysaccharide group, significant higher than that in the three control groups(P〈0.01 ). However, The IL-2 contents in the middle and low dose polysaccharide groups were not found significant difference with the tumor and polysaccharid control groups(P〉0. 05). There were no statistical differences in TNF-α levels in all polysaccharide dose groups( P〉0. 05 ).
Keywords:Apostichopus japonicus  intestinal polysaccharide  H22 tumor bearing mice  antitumor effect
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