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| 叉尾斗鱼遗传多样性的RAPD分析 | |
| 引用本文： | 刘宏毅,黎明星,肖俊,黄愉淋,张明,甘西.叉尾斗鱼遗传多样性的RAPD分析[J].大连水产学院学报,2012,27(2):158-161. |
| 作者姓名： | 刘宏毅 黎明星 肖俊 黄愉淋 张明 甘西 |
| 作者单位： | 1. 广西大学 亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004;广西大学 动物繁殖研究所,广西南宁530004 2. 广西大学 亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004;广西大学 动物繁殖研究所,广西南宁530004;广西水产研究所 广西水产遗传育种与健康养殖重点实验室,广西南宁530021 3. 广西水产研究所 广西水产遗传育种与健康养殖重点实验室,广西南宁,530021 |
| 基金项目： | 广西自然科学基金资助项目，中国博士后科学基金资助项目，广西壮族自治区直属公益性科研院所基本科研业务费资助项目 |
| 摘 要： | 采用RAPD标记技术对叉尾斗鱼Macropodus opercularis 5个地理群体（南宁群体、桂林群体、龙岩群体、福州群体和广州群体）的遗传多样性进行了检测。结果表明：从40个随机引物中筛选出11个有效引物,对每条叉尾斗鱼的基因组DNA进行扩增,共扩增出139条片段;各群体的多态位点比例为26.44%～40.95%,Nei基因多样性指数为0.1042～0.1457,Shannon指数为0.1543～0.2176;而总体的多态位点比例、Nei基因多样性指数和Shannon指数分别高达84.89%、0.3110和0.4606。研究表明,叉尾斗鱼的遗传变异主要来自群体间,而群体内部的遗传多样性水平较低。 |
| 关 键 词： | 叉尾斗鱼 遗传多样性 随机扩增多态DNA |
Analysis of genetic diversity of paradisefish Macropodus opercularis using RAPD markers |
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| LIU Hong-yi , LI Ming-xing , XIAO Jun , HUANG Yu-lin , ZHANG Ming , GAN Xi.Analysis of genetic diversity of paradisefish Macropodus opercularis using RAPD markers[J].Journal of Dalian Fisheries University,2012,27(2):158-161. | |
| Authors: | LIU Hong-yi LI Ming-xing XIAO Jun HUANG Yu-lin ZHANG Ming GAN Xi |
| Institution: | 1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Guangxi University,Nanning 530004,China;2.Animal Reproduction Institute,Guangxi University,Nanning 530004,China;3.Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Guangxi Fisheries Research Institute,Nanning 530021,China) |
| Abstract: | Random amplified polymorphic DNA（RAPD） technique was employed to detect the genetic variations in paradisefish Macropodus opercularis populations distributed in five geographical colonies.In this study,11 effective primers out of 40 random primers were selected to amplify genomic DNA,resulting in 139 repeatable RAPD bands.The 5 populations were shown to have polymorphism frequency of 26.44%-40.95%,Shannon genetic diversity index of 0.1042-0.1457 and Nei genetic diversity of 0.1543-0.2176.On the average,all the population showed the frequency of polymorphism of 84.89%,Nei genetic diversity of 0.3110 and Shannon genetic diversity index of 0.4606,indicating that the genetic variation of the paradiselfish was not from the intra-populations but from inter-populations. |
| Keywords: | Macropodus opercularis genetic diversity random amplified polymorphic DNA（RAPD） |
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