| 大口黑鲈遗传连锁图谱的构建 |
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| 引用本文： | 卢建峰,白俊杰,李胜杰,樊佳佳,徐浩.大口黑鲈遗传连锁图谱的构建[J].大连水产学院学报,2011,26(2):138-143. |
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| 作者姓名： | 卢建峰 白俊杰 李胜杰 樊佳佳 徐浩 |
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| 作者单位： | 中国水产科学研究院珠江水产研究所中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室;上海海洋大学水产与生命学院; |
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| 基金项目： | 国家科技支撑项目(2006BAD01A1209); 农业部公益性行业科研专项项目(200903045); 农业部“948”项目(2010-Z9); 广东省科技计划项目(2007B020708008) |
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| 摘 要： | 采用AFLP技术结合＂拟测交＂策略,以大口黑鲈Micropterus salmoides选育家系作为亲本进行单对杂交产生的F1代家系为作图群体,初步构建了大口黑鲈雌、雄性遗传连锁图谱。结果表明：用64对AFLP引物组合对父母本和106个子代个体进行遗传分析,共得到398个分离标记。母本分离标记有189个,其中172个符合1∶1孟德尔分离规律;父本分离标记有209个,其中181个符合1∶1孟德尔分离标记。雌性框架图包括75个遗传标记,分布在21个连锁群中,有6个三联体,6个连锁对,标记间平均间隔为18.22cM,图谱总长度为983.8 cM;雄性框架图包括82个遗传标记,分布在22个连锁群中,有7个三联体,5个连锁对,标记间平均间隔为19.22 cM,图谱总长度为1153.2 cM。雌、雄性基因组估算长度分别为1 399.85和1 582.72,图谱的总覆盖率分别达到70.28%和72.86%。
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| 关 键 词： | 大口黑鲈 AFLP 遗传连锁图谱 |
Construction of genetic linkage maps in largemouth bass Micropterus salmoides using AFLP markers |
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| LU Jian-feng,BAI Jun-jie,LI Sheng-jie,FAN Jia-jia,XU Hao.Construction of genetic linkage maps in largemouth bass Micropterus salmoides using AFLP markers[J].Journal of Dalian Fisheries University,2011,26(2):138-143. |
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| Authors: | LU Jian-feng BAI Jun-jie LI Sheng-jie FAN Jia-jia XU Hao |
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| Institution: | LU Jian-feng1,2,BAI Jun-jie1,LI Sheng-jie1,FAN Jia-jia1,XU Hao1(1.Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Tropical & Subtropical Fish Breeding & Cultivation,Guangzhou 510380,China,2.College of Life Science and Technology,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | The linkage maps in the broodstock and F1 progeny as segregating population of largemouth bass Micropterus salmoides were primarily constructed using two way pseude testcross strategy with AFLP markers.A total of 2 135 markers were generated in the brood stock and 106 progenies of the mapping family by using 64 AFLP primer combinations,in which 398 markers were found polymorphic between the broodstock.Chi square analysis indicated that among the 189 female and the 209 male segregating markers,172(91.0%) fem... |
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| Keywords: | largemouth bass genetic linkage map AFLP |
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