| 海燕体壁胶原蛋白提取工艺的初步研究 |
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| 引用本文： | 刘远平,张琳琳,张慧怡,赵丹,马然,汪秋宽.海燕体壁胶原蛋白提取工艺的初步研究[J].大连水产学院学报,2011,26(1):54-57. |
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| 作者姓名： | 刘远平 张琳琳 张慧怡 赵丹 马然 汪秋宽 |
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| 作者单位： | 大连海洋大学,辽宁省水产品加工及综合利用重点开放实验室,辽宁,大连,116023 |
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| 摘 要： | 采用两种不同的方法从海燕Asterina pectinifera体壁中提取出酸溶性胶原蛋白（ASC）和胃蛋白酶促溶性胶原蛋白（PSC）。结果表明：ASC的最佳反应条件是,温度为10℃,提取液种类为乙酸-乙酸钠溶液,提取液浓度为0.5 mol/L,提取时间为72 h,ASC得率为7.93%;PSC的最佳反应条件是,温度为17℃、胃蛋白酶酶加量为4%、提取时间为96 h,PSC得率为49.61%。经紫外光谱和氨基酸组成分析,结果表明,ASC和PSC均为典型的Ⅰ型胶原蛋白。
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| 关 键 词： | 海燕 酸溶性胶原蛋白 酶促溶性胶原蛋白 提取工艺 |
Extraction of collagen from body wall of star fish Asterina pectinifera |
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| LIU Yuan-ping,ZHANG Lin-lin,ZHANG Hui-yi,ZHAO Dan,MA Ran,WANG Qiu-kuan.Extraction of collagen from body wall of star fish Asterina pectinifera[J].Journal of Dalian Fisheries University,2011,26(1):54-57. |
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| Authors: | LIU Yuan-ping ZHANG Lin-lin ZHANG Hui-yi ZHAO Dan MA Ran WANG Qiu-kuan |
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| Institution: | LIU Yuan-ping,ZHANG Lin-lin,ZHANG Hui-yi,ZHAO Dan,MA Ran,WANG Qiu-kuan(The Key and Open Laboratory of Fishery Product Processing and Utilization of Liaoning Province,Dalian Ocean University,Dalian 116023,China) |
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| Abstract: | Acid-soluble collagen（ASC） and pepsin-solubilized collagen（PSC） were extracted from body wall of star fish Asterina pectinifera by two different methods.The optimum extraction of ASC with the yield of 7.93% were found at hydrolyzing temperature of 10 ℃,hydrolyzing time of 72 h and the concentration of 0.5 mol/L acetic acid-sodium acetate.The optimum conditions for the extraction of PSC with yield of 49.61% were found as follows：hydrolyzing temperature of 17 ℃,hydrolyzing time of 96 h,and the pepsin dose of 4%.The UV spectra and Amino Acid Analysis revealed that ASC and PSC from body wall of the star fish were classified as type I collagen. |
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| Keywords: | Asterina pectinifera acid-soluble collagen pepsin-solublized collagen extraction |
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