饥饿和再投喂对中间球海胆性腺发育的影响

对中间球海胆Strongylocentrotus intermedius进行了不同时间的饥饿和再投喂试验。采用各组“饥饿时间 投喂时间”相同的试验设计,在30 d内,试验组(S3、S6、S9、S12组)海胆分别饥饿3、6、9和12d后再连续饱食投喂直至试验结束,同时设对照组S0。结果表明:试验结束时S3组海胆性腺重、性腺指数均接近对照组,其余各组较对照组低;特殊生长率在再投喂的第2 d或第4 d出现最大值。性腺组织学观察的结果表明:对照组海胆性腺为滤泡型,由生殖细胞和营养细胞构成,发育至生长期;随着饥饿时间的延长,雄海胆性腺中成熟精子的数量减少,雌海胆性腺中初级卵母细胞发育迟缓,而雌、雄海胆性腺中营养细胞的数量无明显变化;再投喂后,性腺有不同程度的发育。

Effects of starvation and refeeding on gonadal development in sea urchin( Strongylocentrotus internedius)

The 30 day recovery growth experiment was conducted in sea urchin(Strongylocentrotus intermedius) starved for different periods.Groups S0,S3,S6,S9,and S12 were deprived of food for 0,3,6,9 and 12 days,respectively,and then refed at libitum ration levels.In the process,gonad weight,gonado-somatic index and gonad specific growth rate were observed.The results showed that there were significantly lower gonad weight and gonado-somatic index in Groups S6,S9,and S12 than those in Groups S0,and S3.The sea urchin had maximal gonad specific growth rate at 2 or 4 days after they were refed.The histological observation showed that the structure of gonad was follicle type,consisting of germ cells and nutritive cells during growing stage in Group S0.The sea urchin starved had slow germ cell development and no more changes in nutritive cells and continued to develop after refeeding.