| 人工养殖鲶的TRAP遗传分析 |
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| 引用本文： | 葛学亮,孙中武,尹洪滨.人工养殖鲶的TRAP遗传分析[J].大连水产学院学报,2010,25(1):76-79. |
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| 作者姓名： | 葛学亮 孙中武 尹洪滨 |
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| 作者单位： | 1. 东北林业大学,生命科学学院,黑龙江,哈尔滨,150040
2. 中国水产科学研究院,黑龙江水产研究所,黑龙江,哈尔滨,150070 |
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| 基金项目： | 国家基础技术条件平台项目(12006PKA30470-0051) |
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| 摘 要： | 建立了鲶Silurus asotus的TRAP—PCR反应体系，采用24个TRAP引物组合对鲶人工选育群体进行引物适用性的研究，将获得的多态性位点用于研究鲶群体的遗传结构。结果表明：有9个引物组合可产生有效位点，其中多态引物占所筛选引物总数的37．5％，平均每条引物扩增出15个位点。共扩增出135个位点，多态位点数为75，多态位点比例为55．56％。说明TRAP标记技术适用于鲶的遗传多样性研究，可以作为构建鲶遗传连锁图谱的分子标记。
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| 关 键 词： | 鲶 遗传多样性 TRAP 遗传连锁图谱 |
Genetic analysis of cultured catfish Silurus asotus using TRAP technique |
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| GE Xue-liang,SUN Zhong-wu,YIN Hong-bin.Genetic analysis of cultured catfish Silurus asotus using TRAP technique[J].Journal of Dalian Fisheries University,2010,25(1):76-79. |
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| Authors: | GE Xue-liang SUN Zhong-wu YIN Hong-bin |
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| Institution: | 1.College of Life Science;Northeast Forestry Univ.;Harbin 150040;China;2.Heilongjiang River Fishery Research Institute;Chinese Academy of Fishery Sciences;Harbin 150070;China |
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| Abstract: | TRAP(Target Region Amplified Polymorphism) was first used for research of genetic diversity in catfish Silurus asotus.Nine pairs of primers selected from 24 primers produced good amplified patterns with 37.5% of polymorphic primer was in all amplified primers.There were 15 loci amplified by one primer,and 75 polymorphic loci were obtained in all 135 amplified loci with 55.56% of polymorphic loci.The genetic variation analyses showed that TRAP marker can be used in genetic structure research of the catfish.T... |
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| Keywords: | Silurus asotus genetic diversity TRAP genetic linkage map |
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