用黄色隐球酵母发酵生产辅酶Q10最佳条件的选择

从黄色隐球酵母Cryptococcus luteolus L3302中提取辅酶Q10，经过对氮源、碳源、初始pH、发酵温度等的研究分析，得到最佳的发酵条件。通过最优化试验，确定培养基的碳源为蔗糖和葡萄糖各1．25g／L；氮源为酵母膏和玉米浆，各0．3g／L；pH值6．5，温度28℃，接种量5％，500mL锥形瓶中的装液量为50mL；生长因子以蛋白水解液为优。按此发酵条件上罐发酵，发酵液中菌体生长量为13．4g/L，辅酶Q10的产量为18．2mg／L。

Optimum conditions of coenzyme Q10 production by fermentation process for Cryptococcus luteolus

The optimum fermentation conditions of CoQ10 were studied in Cryptococcus luteolus L3302 by orthogonal experiment. The results showed that the optimum fermentation conditions are as the following:carbon sources of glucose 1.25 g/L, and sucrose 1.25 g/L; nitrogen sources of yeast extract 0.3 g/L, and maize liquid 0.3 g/L; initial pH 6.5 and volume of medium 50 mL/500 mL. Under the optimized fermentation-jar conditions, the biomass reached 13.4 g/L, and CoQ10 concentration was 18.2 mg/L.