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荔枝核中活性成分的提取及抗氧化活力研究
引用本文:熊何健,庞杰,林琳.荔枝核中活性成分的提取及抗氧化活力研究[J].西南大学学报,2010,32(3).
作者姓名:熊何健  庞杰  林琳
作者单位:熊何健,XIONG He-jian(福建农林大学食品科学学院,福州,350002;集美大学生物工程学院,福建厦门,361021);庞杰,PANG Jie(福建农林大学食品科学学院,福州,350002);林琳,LIN Lin(集美大学生物工程学院,福建厦门,361021)
基金项目:福建省科技计划重点项目,厦门市科技项目,集美大学创新团队基金
摘    要:对荔枝核中活性成分的提取工艺及提取物抗氧化活性进行了研究.结果表明:荔枝核粉碎过40目筛,以5倍(V/W)70%的丙酮溶液于45℃下浸提2次,每次3 h,浸提液回收溶剂后经AB-8大孔树脂柱层析吸附,用60%乙醇溶液常温洗脱,洗脱速率3 BV/h,洗脱液浓缩、冻干,得荔枝核提取物,得率为11.38%.荔枝核提取物中皂甙含量为61.90%,多酚舍量为27.31%;提取物清除DPPH的IC_(50)值为5.1 mg/L,总抗氧化活力为62.81 U/mg.
Abstract:
The preparation technology and antioxidant activities of the active substances from semen litchi were studied.Semen litchis were ground and then infused twice with 5 volumes(V/W)of 70% acetone so-lution at 45℃ for 3 hr each time.The crude extract was purified with AB-8 type resin, and eluted with 60% ethanol solution under ambient temperature at 3BV/h.The yield of the purified extract was 11.38 %.The contents of saponins and polyphenols were 61.90% and 27.31%, respectively.Its IC50 value on scavenging DPPH radicals and total antioxidant ability were 5.1 mg/L and 62.81 U/mg, respectively.

关 键 词:荔枝核  活性成分  提取工艺  抗氧化活性

Study on the Preparation of Active Substances from Semen Litchi and Their Antioxidant Activity
XIONG He-jian,PANG Jie,LIN Lin.Study on the Preparation of Active Substances from Semen Litchi and Their Antioxidant Activity[J].Journal of Southwest Agricultural University,2010,32(3).
Authors:XIONG He-jian  PANG Jie  LIN Lin
Abstract:
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