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芥菜LFY同源基因克隆及序列分析
引用本文:张敏,汤青林,宋明,王志敏,刘婷.芥菜LFY同源基因克隆及序列分析[J].西南大学学报,2009,31(4).
作者姓名:张敏  汤青林  宋明  王志敏  刘婷
作者单位:西南大学园艺园林学院,重庆市蔬菜学重点实验室,重庆,400716
基金项目:重庆市自然科学基金资助项目
摘    要:在提取芥菜基因组DNA的基础上,克隆了芥菜中的LEAFY(LFY)同源基因,该基因长为1 307 bp.经过对该基因片段的核苷酸序列分析表明,它和花椰菜的BOFH基因在结构上有高度的保守性,同源率高达93%,与拟南芥中的LFY基因同源性达87%.
Abstract:
The homologous DNA fragment of L FY was cloned from mustard genomic DNA that had been isolated from Brassica juncea Coss. Its length was 1307kb. Analysis of the nucleotide sequence indicated that homology was up to 93% between it and BOFH of cauliflower, and up to 87% between it and LFY of Arabidopsis thaliana, which implies that they are highly conserved on their structure.

关 键 词:芥菜  LFY同源基因  克隆

Cloning and Analysis of LFY- like Gene from Brassica juncea Coss.
ZHANG Min,TANG Qing-lin,SONG Ming,WANG Zhi-min,LIU Ting.Cloning and Analysis of LFY- like Gene from Brassica juncea Coss.[J].Journal of Southwest Agricultural University,2009,31(4).
Authors:ZHANG Min  TANG Qing-lin  SONG Ming  WANG Zhi-min  LIU Ting
Abstract:
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