猪链球菌2型CVCC606株免疫相关抗原的纯化及其免疫保护研究

以猪链球菌2型CVCC606免疫断乳仔猪制备高免血清,纯化血清IgG,制备亲和层析柱,亲和纯化其免疫相关抗原;SDS-PAGE分析抗原的组成,研究其对猪链球菌2型CVCC606攻击的BALB/c小鼠的免疫保护作用。结果表明:以亲和层析方法纯化获得的免疫相关抗原分子量集中于29.0~97.2 kD,对5倍LD50剂量的猪链球菌CVCC606攻击的BALB/c小鼠的保护率为60%,呈现了部分的保护作用。所获得的免疫相关抗原中含有保护性抗原。

Studies on Purification of the Immune Related Antigens of Streptococcus suis serotype 2 CVCC606 Strain and Its Immunoprotection

In this study,the SS2 CVCC606 strain was used to immune the post weaning pigs and the immune sera were collected.After the purification of the antibodies from the sera,an affinity chromatographic column was prepared with the IgGs as affinity ligands used to purify the immune related antigens of the SS2 CVCC606 strain.The antigens were analyzed by SDS-PAGE and immune to the BALB/c mice to study the effect of protection.The results suggest that the molecular weight of the antigens purified with the SS2 CVCC606 strain immune sera were mainly distributed from 29.0 kD to 97.2 kD.There are only 60% BALB/c mice immune with the purified antigens survived when challenged with CVCC 606 strain at 5 folds of the LD50.A partly protection was obtained in mice and the immune related antigens purified in this study contained the protective compositions.