| 利用表面等离子共振技术检测牛奶中的朊蛋白 |
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| 引用本文： | 王雄,万家余,李吉平,徐静,吴松波,高宏伟.利用表面等离子共振技术检测牛奶中的朊蛋白[J].吉林农业大学学报,2010,32(4):433-436. |
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| 作者姓名： | 王雄 万家余 李吉平 徐静 吴松波 高宏伟 |
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| 作者单位： | 军事医学科学院军事兽医研究所,长春,130062 |
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| 基金项目： | 国家自然科学基金项目 |
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| 摘 要： | 利用生物传感器——表面等离子共振传感器建立在活体动物中检测朊蛋白的方法,依据抗原—抗体特异性原理检测牛奶中朊蛋白。结果表明:应用表面等离子共振技术进行检测,1 h以内即可达到在缓冲液中标准最低检测限30 ng/mL的水平,牛奶样品的最低检测限是120 ng/mL。
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| 关 键 词： | 朊蛋白 表面等离子共振 牛奶 |
Detection of Prion Protein in Milk Using Surface Plasmon Resonance Technique |
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| WANG Xiong,WAN Jia-yu,LI Ji-ping,XU Jing,WU Song-bo,GAO Hong-wei.Detection of Prion Protein in Milk Using Surface Plasmon Resonance Technique[J].Journal of Jilin Agricultural University,2010,32(4):433-436. |
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| Authors: | WANG Xiong WAN Jia-yu LI Ji-ping XU Jing WU Song-bo GAO Hong-wei |
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| Institution: | Institute of Veterinary, Academy of Military Medical Sciences, Changchun 130062,China |
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| Abstract: | The health of human and animal is threatened by transmissible spongiform encephalopathis. The purpose of this study is to develop a detection of prionprotein in live animal with surface plasmon resonance technique. This detectionis label free, real time and convenient. It was demonstrated that the sensor is capable ofdetecting concentrations of prion protein as low as 30 ng／mL and 120 ng／mL in buffer and milk respectively, but only one hour at most. |
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| Keywords: | milk |
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