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| 体外培养枫径猪胚胎成纤维细胞系方法的建立 | |
| 引用本文： | 陈颖,逢大欣,车东升,赵增明,赖良学,欧阳红生.体外培养枫径猪胚胎成纤维细胞系方法的建立[J].吉林农业大学学报,2007,29(3):310-314. |
| 作者姓名： | 陈颖 逢大欣 车东升 赵增明 赖良学 欧阳红生 |
| 作者单位： | 1. 吉林大学畜牧兽医学院,长春,130062;吉林农业大学参茸中心,长春,130118 2. 吉林大学畜牧兽医学院,长春,130062 3. 吉林大学畜牧兽医学院,长春,130062;吉林农业大学科技出版部,长春,130118 4. 军事医学科学院,北京,100071 |
| 基金项目： | 国家自然科学基金资助项目(30070563)，海外青年学者合作项目(30428027) |
| 摘 要： | 选取枫径猪胚胎,采用胶原酶消化培养法制备猪胚胎成纤维细胞,经数次传代培养及冷冻复苏试验表明胚胎成纤维细胞仍具有正常的传代能力。在体外培养至15代后成纤维细胞核型正常,符合体细胞克隆转基因的要求。用PCR方法建立了枫径猪胚胎成纤维细胞性别鉴定的反应体系,对其中6株细胞进行了性别鉴定。 |
| 关 键 词： | 枫径猪 胚胎成纤维细胞 体外培养 |
| 文章编号： | 1000-5684（2007）03-0310-05 |
| 修稿时间： | 2006-08-202006-11-20 |
Establishment of A Method for Fengjing Porcine Fetal Fibroblast Cell Line in Vitro |
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| CHEN Ying,PANG Da-xin,CHE Dong-sheng,ZHAO Zeng-ming,LAI Liang-xue,OUYANG Hong-sheng.Establishment of A Method for Fengjing Porcine Fetal Fibroblast Cell Line in Vitro[J].Journal of Jilin Agricultural University,2007,29(3):310-314. | |
| Authors: | CHEN Ying PANG Da-xin CHE Dong-sheng ZHAO Zeng-ming LAI Liang-xue OUYANG Hong-sheng |
| Institution: | 1. College of Animal Husbandry and Veterinary Medicine, Jilin University, Changchun 130062, China; 2. Center of Ginseng and Antd, Jilin Agricultural University, Changehun 130118, China; 3. Publishing Department of Science and Technology, Jilin Agricultural University, Changehun 130118, China ; 4. Academy of Military Medical Sciences, Beijing 100071, China |
| Abstract: | Fengjing porcine fetal fibroblasts were cultured by fetal tissue pieces which were digested with collagenase.and an ideal cellular culture method was established.After several pasages of culture and freeze revival,the fetal fibroblast cells still had normal capacity of passage.Karyotype analysis indicated that after 15 passages of in vitro culture they showed no abnormal appearance.It suits for the need of cloning and transgenic construction.After establishing the technique of sex determination of fetal fibroblast of porcine,we identified the sex of 6 cell lines. |
| Keywords: | Fengjing pig fetal fibroblast cell in vitro culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |