|
|||||
|
|
| 蓖麻毒素B链(RTB)酵母双杂交诱饵载体的构建及其自激活作用的检测 | |
| 引用本文： | 刘宁,刘林娜,秦贵信,徐静,万家余,高宏伟.蓖麻毒素B链(RTB)酵母双杂交诱饵载体的构建及其自激活作用的检测[J].吉林农业大学学报,2010,32(4):398-401. |
| 作者姓名： | 刘宁 刘林娜 秦贵信 徐静 万家余 高宏伟 |
| 作者单位： | 1. 吉林农业大学动物科技学院,长春130118;军事医学科学院军事兽医研究所,长春130062 2. 军事医学科学院军事兽医研究所,长春,130062 3. 吉林农业大学动物科技学院,长春,130118 |
| 基金项目： | 全军医药卫生科研基金项目 |
| 摘 要： | 采用PCR技术扩增蓖麻毒素B链基因,并克隆入诱饵载体pSos中,成功地构建了酵母双杂交诱饵载体pSos-RTB。将重组质粒与对照质粒共转化入酵母菌cdc25H。结果表明:该段基因表达的蛋白对酵母菌cdc25H无毒性和无自激活作用,且在酵母细胞中定位正确。 |
| 关 键 词： | 蓖麻毒素蛋白 酵母双杂交系统 诱饵载体 自激活 |
Construction of Ricin-B (RT-B) Gene Bait Vector and Verification of Self-Activation in Yeast Double-Hybrid System |
|
| LIU Ning,LIU Lin-na,QIN Gui-xin,XU Jing,WAN Jia-yu,GAO Hong-wei.Construction of Ricin-B (RT-B) Gene Bait Vector and Verification of Self-Activation in Yeast Double-Hybrid System[J].Journal of Jilin Agricultural University,2010,32(4):398-401. | |
| Authors: | LIU Ning LIU Lin-na QIN Gui-xin XU Jing WAN Jia-yu GAO Hong-wei |
| Institution: | 1.College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China|2.Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130062, China |
| Abstract: | The ricin B gene was cloned into the pSos vector. After being verified by restriction enzyme digestion analysis, the recombinant bait vector pSos RTBof yeast double hybrid system was obtained. Bait and target plasmids were cotransformed into the cdc25H yeast strain. This result means it was not toxic to cdc25H and had no self activation. |
| Keywords: | self activation |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《吉林农业大学学报》浏览原始摘要信息 | |
| 点击此处可从《吉林农业大学学报》下载免费的PDF全文 | |