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| 纳豆激酶基因的克隆及表达研究 | |
| 引用本文： | 姜媛媛,王曙文,王铁东,丁东红,王景会.纳豆激酶基因的克隆及表达研究[J].吉林农业大学学报,2009,31(4). |
| 作者姓名： | 姜媛媛 王曙文 王铁东 丁东红 王景会 |
| 作者单位： | 1. 中国农业科技东北创新中心农产品加工研究中心,长春,130033 2. 吉林大学农学部,长春,130062 3. 吉林大学第四医院-一汽总医院,长春,130011 4. 中国农业科技东北创新中心农产品加工研究中心,长春,130033;吉林农业大学食品科学与工程学院,长春,130118 |
| 基金项目： | 吉林省科技发展计划项目 |
| 摘 要： | 经PCR扩增获得了纳豆激酶基因(NK).将此基因插入原核表达载体pTWIN1中,使之与几丁质结合域(CBD)一内含肽(intein)融合,获得原核表达质粒pTWIN1/NK.转化宿主菌E.coli BL21(DE3),在IPTG诱导下进行纳豆激酶的表达研究.SDS-PAGE电泳分析结果表明,重组蛋白在BL21(DE3)中获得高效表达,在低温诱导时主要以可溶性蛋白的形式存在. |
| 关 键 词： | 纳豆激酶基因 克隆 表达 |
Cloning and Expression of the Nattokinase Gene |
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| JIANG Yuan-yuan,WANG Shu-wen,WANG Tie-dong,DING Dong-hong,WANG Jing-hui.Cloning and Expression of the Nattokinase Gene[J].Journal of Jilin Agricultural University,2009,31(4). | |
| Authors: | JIANG Yuan-yuan WANG Shu-wen WANG Tie-dong DING Dong-hong WANG Jing-hui |
| Abstract: | Nattokinase gene was amplified using polymerase chain reaction(PCR),and cloned into prokaryotic expression vector pTWIN1.The expression plasmids of pTWIN1/NK was constructed and was further transformed into E.coli BL21(DE3).Expression of the enzyme was performed at different temperatures with IPTG induction.SDS-PAGE showed that nattokinase was expressed in soluble form at a low temperature.The cloning and expression of nattokinase would be helpful in producing nattokinase by gene engineering strain. |
| Keywords: | nattokinase gene cloning expression |
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