| 笃斯越橘CBF基因的克隆及序列分析 |
| |
| 引用本文： | 臧建磊,李亚东,刘庆忠,宗晓娟,王甲威.笃斯越橘CBF基因的克隆及序列分析[J].吉林农业大学学报,2011,33(5):532-535. |
| |
| 作者姓名： | 臧建磊 李亚东 刘庆忠 宗晓娟 王甲威 |
| |
| 作者单位： | 1. 吉林农业大学园艺学院,长春,130118
2. 山东省果树研究所,秦安271000;山东省果树生物技术育种重点实验室,泰安271000 |
| |
| 基金项目： | 农业部公益性行业(农业)科研专项(201103037) |
| |
| 摘 要： | 根据近缘植物中同源CBF基因的序列保守区设计引物,采用PCR方法,克隆出野生种笃斯越橘的CBF基因片段,将其克隆到pMD18-T Vector上.序列分析结果表明,笃斯越橘的CBF基因序列全长678 bp,编码225个氨基酸.同源性分析表明该基因的核苷酸序列和推导的氨基酸序列与其他植物CBF类基因具有较高的同源性,与桃...
|
| 关 键 词： | 笃斯越橘 CBF转录因子 克隆 |
Cloning and Sequence Analysis of CBF gene in Blueberry(Vaccinium uliginosuml L.) |
| |
| ZANG Jian-lei,LI Ya-dong,LIU Qing-zhong,ZONG Xiao-Juan,WANG Jia-wei.Cloning and Sequence Analysis of CBF gene in Blueberry(Vaccinium uliginosuml L.)[J].Journal of Jilin Agricultural University,2011,33(5):532-535. |
| |
| Authors: | ZANG Jian-lei LI Ya-dong LIU Qing-zhong ZONG Xiao-Juan WANG Jia-wei |
| |
| Institution: | 1.College of Horticulture,Jilin Agricultural University,Changchun 130118,China;2.Shandong Institute of Pomology,Tai′an 271000,China;3.Shandong Key Laboratory of Fruit Biotechnology Breeding,Tai′an 271000,China |
| |
| Abstract: | Primers were designed according to the sequence of CBF gene in other plants.Fragment of CBF gene was isolated from Blueberry(Vaccinium uliginosuml L.) by PCR,and transferred into the pMD18-T Vector.The result derived from the sequence analysis showed that the sequences obtained the full-length cDNA was 678 bp long,and encoded a putative protein of 225 amino acids.Homology analysis showed that the nucleotide and the deduced amino acids sequences were homologous to the CBF genes in other species,especially st... |
| |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
