| 豌豆凝集素基因转化单倍体烟草 |
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| 引用本文： | 王逸群,孙珊珊,赵仁贵,王玉兰.豌豆凝集素基因转化单倍体烟草[J].吉林农业大学学报,2001,23(2):21-23. |
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| 作者姓名： | 王逸群 孙珊珊 赵仁贵 王玉兰 |
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| 作者单位： | 1. 福建师范大学生物工程学院,
2. 吉林农业大学农学院, |
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| 基金项目： | 国家自然科学基金资助项目 ( 395 70 194) |
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| 摘 要： | 豆科植物凝集素在对根瘤菌的识别中起重要作用。通过农杆菌介导，采用叶盘法将豌豆凝集素基因转化单倍体烟草，获得了转基因再生植株。对转基因植株进行了GUS检测。该转基因单倍体烟草可用于非豆科植物进凝集素对根瘤菌的识别作用研究。
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| 关 键 词： | 豌豆凝集素基因转化 单倍体烟草 基因转化 生物固氮 根瘤菌 |
| 文章编号： | 1000-5684（2001）02-0021-03 |
| 修稿时间： | 2000年9月28日 |
Transformation of Pea Lectin Gene Into Haploid Tobacco |
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| Wang Yi-qun,SUN Shan-shan,ZHAO Ren-gui,WANG Yu-lan.Transformation of Pea Lectin Gene Into Haploid Tobacco[J].Journal of Jilin Agricultural University,2001,23(2):21-23. |
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| Authors: | Wang Yi-qun SUN Shan-shan ZHAO Ren-gui WANG Yu-lan |
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| Institution: | WANG Yi qun 1,ZHAO Ren gui 2,WANG Yu lan 2,SUN Shan shan 1 |
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| Abstract: | The leguminous lectin played an important role in the recognition of Rhizobia . The pea lectin gene was transformed into the haploid tobacco via Agrobacterium mediation using leaf disc method and the regenerated tobacco plants were obtained. Histochemical staining of GUS activity was made in the transgenic plants. This plants could be used in researches on the recognition of Rhizobia in the non legumes. |
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| Keywords: | pea lectin transformation gene haploid tobacco |
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