| 一种对固氮细菌感染植物进行显微观察的改良方法 |
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| 引用本文： | 王逸群,赵仁贵,王玉兰,李文明,马杰.一种对固氮细菌感染植物进行显微观察的改良方法[J].吉林农业大学学报,2008,30(5). |
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| 作者姓名： | 王逸群 赵仁贵 王玉兰 李文明 马杰 |
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| 作者单位： | 1. 福建师范大学生命科学学院发育与神经生物学重点实验室,福州,350108
2. 吉林农业大学农学院,长春,130118 |
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| 基金项目： | 福建省科技厅重点科技项目，福建省自然基金项目，福建省教育厅科技项目 |
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| 摘 要： | 介绍了一种对固氮细菌感染植物进行显微观察的改良方法--共聚焦激光扫描显微镜三通道扫描法.该方法与常规的光学显微镜观察、电子显微镜观察相比,具有操作简便、不需要繁琐的前处理、所用仪器少、有效可行等特点,而且在有些应用共聚焦激光扫描显微镜双通道法观察固氮细菌对植物的感染效果不理想的情况下,可以采用共聚焦激光扫描显微镜三通道扫描法观察.
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| 关 键 词： | 固氮细菌 感染 植物 显微观察 改良方法 |
A Modified Method for the Observation of the Infection of Diazotrophic Bacteria to Plants |
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| WANG Yi-qun,ZHAO Ren-gui,WANG Yu-lan,LI Wen-ming,MA Jie.A Modified Method for the Observation of the Infection of Diazotrophic Bacteria to Plants[J].Journal of Jilin Agricultural University,2008,30(5). |
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| Authors: | WANG Yi-qun ZHAO Ren-gui WANG Yu-lan LI Wen-ming MA Jie |
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| Institution: | 1.The Key Laboratory of the University in Fujian for Developmental and Neural Biology;College of Life Sciences;Fujian Normal University;Fuzhou 350108;China;2.College of Agronomy;Jilin Agricultural University;Changchun 130118;China |
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| Abstract: | This paper introduces a modified method for the observation of the infection of diazotrophic bacteria to plants,i.e.the method for its observation under the confocal laser scanning microscope(CLSM) in three channels.In comparison with the traditional methods for its observation under the light microscope and electron microscope,it has many advantages.First of all,the operation is easy;second,the preparation of the samples is not complicated;third,it has a small need for instrument;fourth,it is effective and... |
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| Keywords: | diazotrophic bacteria infection plants observation under the microscope the modified method |
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