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| 桦褐孔菌菌丝液体培养条件的研究 | |
| 引用本文： | 陈艳秋,李玉,周丽洁.桦褐孔菌菌丝液体培养条件的研究[J].吉林农业大学学报,2006,28(4):358-360,368. |
| 作者姓名： | 陈艳秋 李玉 周丽洁 |
| 作者单位： | 1. 吉林农业大学菌物所,长春,130118;延边大学农学院园艺系,龙井,133400 2. 吉林农业大学菌物所,长春,130118 3. 延边大学农学院园艺系,龙井,133400 |
| 基金项目： | 吉林省教育厅科研项目;延边大学校科研和教改项目 |
| 摘 要： | 试验研究了碳源、氮源、琼脂、天然物质在桦褐孔菌菌丝体液体培养基中的配比。结果表明:培养基配方以葡萄糖20.0 g/L、淀粉50.0 g/L、蛋白胨3.5 g/L、KH2PO43.0 g/L、MgSO41.5 g/L为最佳,菌丝生物量达0.181 2 g/100 mL。在最佳培养基配方基础上,桦褐孔菌菌丝体摇床培养5 d、培养液装量为容器的1/3时菌丝体生物量达到最大值,为0.041 4 g/50 mL。 |
| 关 键 词： | 桦褐孔菌 菌丝 液体培养 |
| 文章编号： | 1000-5684（2006）04-0358-03 |
| 收稿时间： | 2005-12-28 |
| 修稿时间： | 2005-12-282006-02-22 |
Study on the Conditions of Liquid Culture of Inonotus obliquus |
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| CHEN Yan-qiu,LI Yu,ZHOU Li-jie.Study on the Conditions of Liquid Culture of Inonotus obliquus[J].Journal of Jilin Agricultural University,2006,28(4):358-360,368. | |
| Authors: | CHEN Yan-qiu LI Yu ZHOU Li-jie |
| Institution: | 1. Institute of Mycology, Jilin Agricultural University, Changchun 130118, China ; Horticulture, Agricultural College of Yanbian University, Longjing133400, China |
| Abstract: | The effects of different carbon sources,nitrogen sources,agar,natural substance,culture time and volume of culture solution on liquid culture of Inonotus obliquus were studied.The results indicated that the most suitable culture medium was glucose 20.0 g/L,starch 50.0 g/L,peptone 3.5 g/L,KH_2PO_4 3.0 g/L and MgSO_4 1.5 g/L.The biomass of dry mycelium was 0.181 2 g/100mL.When the culture medium was optimum,culture time was 5 days,and the volume of culture solution was 1/3,the biomass of mycelium reached the highest,0.041 4 g/50mL |
| Keywords: | Inonotus bliquus mycilium liquid culture |
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