西洋参特有成分--拟人参皂苷F11的分离、鉴定与含量测定

为了筛选西洋参的特有成分，以利对西洋参生药及其制品的定性鉴别和含量测定，为研制开发新药提供理论依据，本试验采用大孔树脂法和硅胶柱层析分离法对西洋参甲醇提取物进行分离纯化，将所得单体应用质谱、^13C-核磁共振谱、电子轰击质谱（EL-MS）鉴定，并用高效薄层层析法比较了人参、西洋参和三七的甲醇提取物，用薄层扫描法对西洋参各部位拟人参皂苷F11的含量进行了测定。结果表明：西洋参特有皂苷成分为拟人参皂苷F11,在西洋参的花蕾、花柄、果肉、茎叶和根中的含量依次为2．34％，1．93％，1．54％，0．97％和0．28％。

Isolation, Identification and Content Determination of Pseudogisenoside F11 in American Ginseng

Comparative studies of saponin in tri species plant root of Panax ginseng, Panax quenquifoliurn and Panax notoginseng were conducted. To screen the unique saponin components of Panax quenquifolium which will benefit the qualitative identification and content determination of its biochemical medicines, to provide the theoretical foundation for the development of new medicines, methanol extracts of Panax quenquifolium were isolated, purified and identified. HTLC on methanol extracts of root in Panax ginseng and that in Panax notoginseng were compared. Its isolation was achieved by using polypore resin column and silica gel column chromatography. The identification on unique saponin was made by LCQ-MS,^13C - NMR and acetyl-compound EL- MS. The results showed that the unique saponine was pseudoginsenoside F11, the content of which in flower stem, fruit, leaf and root were respectively 2.34%, 1.93%, 1.54% ,0.97% and 0.28%.