| 菌株MY02发酵培养基的优化设计 |
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| 引用本文： | 刘秋,闫建芳,艾勇,于基成,杨宝灵,范圣第.菌株MY02发酵培养基的优化设计[J].吉林农业大学学报,2006,28(4):361-364. |
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| 作者姓名： | 刘秋 闫建芳 艾勇 于基成 杨宝灵 范圣第 |
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| 作者单位： | 大连民族学院生物工程研究中心,大连,116600 |
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| 基金项目： | 辽宁省自然科学基金;辽宁省教育厅资助项目 |
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| 摘 要： | 以龟裂链霉菌MY02菌株为试材,采用单因子试验与均匀试验相结合的方法,通过二次多项式回归分析,筛选出活性组分SN06最佳发酵培养基配方,并建立了多元二次回归数学模型。菌株MY02优化发酵培养基的最佳配方:淀粉质量分数为2.69%,花生饼粉质量分数为1.39%,(NH4)2SO4质量分数为0.18%,CaCO3质量分数为0.14%,NaCl质量分数为0.16%。根据回归模型计算出SN06理论效价与实测效价相比较,二者非常接近,拟合误差小。
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| 关 键 词： | 龟裂链霉菌 农用抗生素 均匀设计试验 发酵条件 |
| 文章编号： | 1000-5684（2006）04-0361-04 |
| 收稿时间： | 2005-07-07 |
| 修稿时间： | 2005-07-072005-12-10 |
Optimization of Fermentation Culture Medium of Isolate MY02 |
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| LIU Qiu,YAN Jian-fang,Ai Yong,YU Ji-cheng,YANG Bao-ling,FAN Sheng-di.Optimization of Fermentation Culture Medium of Isolate MY02[J].Journal of Jilin Agricultural University,2006,28(4):361-364. |
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| Authors: | LIU Qiu YAN Jian-fang Ai Yong YU Ji-cheng YANG Bao-ling FAN Sheng-di |
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| Institution: | Research Center of Biotechnology, Dalian Nationalities University, Dalian 116600, China |
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| Abstract: | Active components SN06 in fermentation of Streptomyces rimosus MY02 show antagonism against pathogen.It is a basic implementation for production of SN06 that screens medium ingredients and corresponding dosage.In this study,the optimum medium ingredients and dosage of Streptomyces rimosus MY02 were determined using uniform design combined with regression analysis. A regression model was developed.The optimum medium was starch 2.69%,peanut steep powder 1.39%,(NH_4)_2SO_4 0.18%,CaCO_3 0.14% and NaCl 0.16%.The regression model was tested with titre of SN06.The tested result fitted well with those calculated with the model.The results confirmed the applicability of uniform design for screening the best medium ingredients for SN06. |
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| Keywords: | Streptomgces rimosus agricultural antibiotic uniform design experiment fermentation condition |
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