| 鸡p15INK4B基因的克隆表达及纯化 |
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| 引用本文： | 艾永兴,张玉静,胡薇,郝军元,邹亚学,张英波.鸡p15INK4B基因的克隆表达及纯化[J].吉林农业大学学报,2006,28(4):453-457,461. |
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| 作者姓名： | 艾永兴 张玉静 胡薇 郝军元 邹亚学 张英波 |
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| 作者单位： | 1. 吉林大学畜牧兽医学院,长春,130062
2. 吉林农业大学生物技术学院,长春,130118 |
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| 摘 要： | 为研究鸡p15INK4B蛋白的功能及为制备此蛋白的单克隆抗体提供材料,应用引物搭桥法合成p15INK4B基因,应用基因工程的手段构建p15INK4B基因的原核表达载体,在E.coliRosetta(DE3)菌进行融合表达,并对表达产物进行亲和层析纯化。结果成功地构建了p15INK4B基因原核表达载体,经亲和层析制备了较高纯度的目的表达产物。
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| 关 键 词： | p15INK4B 细胞周期 原核表达 亲和层析 |
| 文章编号： | 1000-5684（2006）04-0453-05 |
| 收稿时间： | 2005-12-27 |
| 修稿时间： | 2005-12-272006-04-20 |
Clone Expression and Purification of p15INK4B Gene of Chicken |
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| Ai Yong-xing,ZHANG Yu-jing,HU Wei,HAO Jun-yuan,ZOU Ya-xue,ZHANG Ying-bo.Clone Expression and Purification of p15INK4B Gene of Chicken[J].Journal of Jilin Agricultural University,2006,28(4):453-457,461. |
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| Authors: | Ai Yong-xing ZHANG Yu-jing HU Wei HAO Jun-yuan ZOU Ya-xue ZHANG Ying-bo |
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| Institution: | 1. College of Animal Husbandry and Veterinary Medicine, Jilin University, Changchun 130062, China ; 2. College of Biotechnology , Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | To study p15~(INK4B) protein and prepare its monoclonal antibody,p15~(INK4B) gene was obtained by overlap-ligation PCR and cloned into expression vector.Then p15~(INK4B) gene was expressed in E.coli Rosetta(DE3) and p15~(INK4B) fusion protein was purified by affinity chromatography.The results indicated that the recombinant expression vector pET28a( )-p15 was successfully constructed and relatively pure target protein was obtained. |
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| Keywords: | p15~(INK4B) cell cycle prokaryotic expression affinity chromatography |
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