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双糖对冻融后猪精子质量的影响
引用本文:韩明铭,金一,方南洙,朴海仙,曹丽朋.双糖对冻融后猪精子质量的影响[J].吉林农业大学学报,2010,32(1):86-88.
作者姓名:韩明铭  金一  方南洙  朴海仙  曹丽朋
作者单位:延边大学农学院,龙井,133400
基金项目:吉林省教育厅项目(吉教科合字[2007]第20号)
摘    要:在传统的Tris—柠檬酸—葡萄糖(TCG)稀释液基础上,分别添加0.1 mol/L乳糖、蔗糖、海藻糖,并采用细管法对精液进行冷冻,研究不同双糖对冻融后猪精液质量的影响。结果表明:0.1 mol/L的海藻糖和蔗糖相对于对照组和0.1 mol/L的乳糖TCG稀释液能够显著改善冻融后猪精子质量,其冻融后精子的活率、顶体完整率、低渗膨胀率、平均路径速度、运动线速度、平均侧摆幅度和平均鞭打频率均明显提高(P0.05),分别达到42.14%和40.56%,51.48%和49.84%,46.20%和43.01%,32.8μm/s和33.2μm/s,60.80%和59.00%,1.70μm和1.62μm,7.68 Hz和7.62 Hz。TCG稀释液中添加0.1 mol/L海藻糖和蔗糖的猪冷冻精液体外受精的受精能力明显高于0.1 mol/L乳糖组和对照组,其卵裂率分别为41%和38%。

关 键 词:猪精子  双糖  冻融  质量参数
收稿时间:2008-10-05
修稿时间:2008-12-18

Effect of Disaccharide on Cryopreservation of Quality Parameters in Boar Sperm
HAN Ming-ming,JIN Yi,FANG Nanzhu,PIAO Hai-xian,CAO Li-peng.Effect of Disaccharide on Cryopreservation of Quality Parameters in Boar Sperm[J].Journal of Jilin Agricultural University,2010,32(1):86-88.
Authors:HAN Ming-ming  JIN Yi  FANG Nanzhu  PIAO Hai-xian  CAO Li-peng
Institution:College of Agriculture, Yanbian University, Longjing 133400, China
Abstract:In order to improve boar sperm quality parameters in frozen-thawed process,0.1 mol/L lactose,sucrose and trehalose were respectively added into the basic extenders Tris-Citric acid-Glucose(TCG).The effects of disaccharide on quality of cryopreservation of boar sperm were investigated.We found out that TCG extender with 0.1 mol/L trehalose and sucrose significantly enhanced motility,acrosome integrity rate,hypotonic expansion rate, VAP, LIN, ALH and BCF of boar sperm after freezing and thawing(P<0.05),they were 42.14% and 40.56%,51.48% and 49.84%,46.20% and 43.01%,32.8 μm/s and 33.2 μm/s,60.80% and 59.00%,1.70 μm and 1.62 μm,7.68 Hz and 7.62 Hz.The IVF rate of frozen-thawed sperm in 0.1 mol/L trehalose and sucrose is significantly higher than that of others(P<0.05). They were 41% and 38%.
Keywords:boar sperm  disaccharide  cryopreservation  quality parameter  
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