Agrobacterial transformation in rape by the use of technology without the phase of regenerating plants from callus

Proposed changes in technology of performing the transformation in dicotyledonous plants were aimed to exclude the phase of regeneration. The seeds of transformed plants were used as recipients, while the appearance of transgenic variants occurs simultaneously with the formation of the first seed progeny T₁. The proposed technology completely lacks any phases connected with the necessity of cultivating some tissues in vitro, which permits one to avoid somaclonal variance with uncontrolled deviations from the initial genotype.