鼓槌石斛组培快繁技术体系研究

以鼓槌石斛八成熟蒴果为外植体,通过对其种子无菌萌发培养、原球茎继代增殖及生根壮苗培养基的筛选,建立鼓槌石斛的组培快繁技术体系。结果表明:1/2 MS+1.0 mg/L NAA+8.0%土豆泥+0.10%活性炭培养基效果较理想,种子萌发率较高,发芽快;最适增殖培养基为3/4 MS+3.0 mg/L 6-BA+8%香蕉泥,增殖系数可达7.23;最佳生根培养基为3/4 MS+1.0 mg/L NAA+0.5 mg/L CA+8%香蕉泥,平均根数量达7.49条。炼苗移栽到松树皮作为栽培基质的苗床上,1个月以后,成活率均达80%。

Tissue Culture-based Rapid Propagation of Dendrobium chrysotoxum Lindl

Immature seeds of Dendrobium chrysotoxum Lindl were used as explants for tissue culture,and mediums for aseptic seed germination,subculture of protocomb,rooting and growth of strong plantlets were screened to establish a rapid propagation system for D.chrysotoxum lindl.The results showed that the medium 1/2 MS +1.0 mg/L NAA+8.0％ mashed potato+1.0％ activated carbon was optimum for seed germination,under which the seeds germinated fast with a higher germination rate.The optimum subculture medium was 3/4 MS+6-BA 3.0 mg/L +8％ mashed banana,which gave a proliferation coefficient of 7.23.The optimum rooting medium was 3/4 MS +NAA 1.0 mg/L +CA 0.5 mg/L+8％ mashed banana,and it produced 7.49 roots by average.The rooted plants were transferred to the seedbed with pine bark as the culture substrate and had a survival rate of 80％ one month after transferring.