海南地不容中总生物碱的提取及含量测定

采用正交试验法优选海南地不容的提取工艺并进行含量测定。以提取的总生物碱为考察指标,醇的体积分数、提取功率、提取时间、次数、料液比分别为考察因素,设计正交试验确定超声提取总生物碱的最优工艺。以盐酸小檗碱为对照品,利用分光光度法在418 nm处测定海南地不容中总生物碱的含量。最佳工艺为醇体积分数80%、超声提取30 min、功率160 W、料液体积比1∶20、提取2次。小檗碱在0.0~18.0μg/mL范围内线性关系良好(r=0.999 4)。平均回收率为99.03%,RSD=2.38%(n=6)。总生物碱含量可达到4.67%。确定的提取工艺简单,用时少,提取率高。建立的测量方法简单、灵敏度高、重现性好,适用于海南地不容中总生物碱的含量测定。

Extraction and Determination of Total Alkaloids from Stephania hainanensis

An orthogonal experiment was designed to screen an optimum method for extraction of total alkaloids from Stephania hainanensis. The total alkaloids extracted was used as an index, and ethanol volume fraction, extraction time, altrasonic power, extraction frequency were used as factors to design the orthogonal experiment to optimize the altrasonic extraction of the total alkaloids from S. hainanensis. The content of total alkaloids extracted was determined by using spectrophotometry at 418 nm with berberine hydrochloride as a reference substance. The extraction was identified optimum when the volume fraction of ethanol was 80%, the extraction time was 30 min, the altrasonic power was 160 W, the solid-liquid ratio was 1:20, and the extraction frequency was 2 times. Berberine hydrochloride had a good linear relationship (r=0.9994) at the range of 0~18.0 μg/mL. The average recovery was 99.03% (RSD=2. 38%). The content of total alkaloids extracted was 4.67%. This extraction technology was proved to be simple, time saving and higher in extraction rate. The determination method for total alkaloids was easy, highly sensitive and good in repeatability, and can be used for determination of the content of total alkaloids from S. hainanensis.