| 几种淡水鱼肌肉乙酰胆碱酯酶的盐析提取及农药敏感性研究 |
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| 引用本文： | 丁运华,严松溪,谢汝朋,黄彬亮.几种淡水鱼肌肉乙酰胆碱酯酶的盐析提取及农药敏感性研究[J].热带农业科学,2011,31(6):21-23. |
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| 作者姓名： | 丁运华 严松溪 谢汝朋 黄彬亮 |
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| 作者单位： | 惠州学院生命科学系,广东惠州,516007 |
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| 基金项目： | 惠州市科技计划项目(2008P60) |
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| 摘 要： | 以鲢鱼、镛鱼和罗非鱼肌肉组织为材料,研究乙酰胆碱酯酶AChE的盐析提取方法及农药敏感性。结果表明,3种淡水鱼肌肉组织在55%硫酸铵饱和度时得到的沉淀酶总活力最高,乙酰胆碱酯酶的比活力分别达到0.264,0.263,0.221 U/mg,纯化倍数分别为1.222,1.277,1.190,回收率分别为27.626%,37.621%,26.441%。5种有机磷农药（敌敌畏,辛硫磷,马拉硫磷,乙酰甲胺磷,氧化乐果）对3种淡水鱼肌肉乙酰胆碱酯酶有抑制作用,其中罗非鱼AChE对敌敌畏最敏感,抑制中浓度I50为0.14μg/mL,对马拉硫磷最不敏感,抑制中浓度I50为11.202μg/mL
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| 关 键 词： | 罗非鱼 乙酰胆碱酯酶 纯化 凝胶过滤 离子交换 农药敏感性 |
Extraction and Insecticides Susceptibility of Acetylcholinesterase from Muscle Tissues of Fresh Water Fishes |
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| DING Yunhua YAN Songxi XIE Rupeng HUNAG Binliang.Extraction and Insecticides Susceptibility of Acetylcholinesterase from Muscle Tissues of Fresh Water Fishes[J].Chinese Journal of Tropical Agriculture,2011,31(6):21-23. |
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| Authors: | DING Yunhua YAN Songxi XIE Rupeng HUNAG Binliang |
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| Institution: | DING Yunhua YAN Songxi XIE Rupeng HUNAG Binliang(Life Science Department,Huizhou University,Huizhou,Guangdong 516007) |
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| Abstract: | Acetylcholinesterase（AChE） from muscle tissues of Hypophthalmichthys molitrix,Aristichthys nobilis and Oreochromis aurea were extracted by（NH4）2SO4 fractional precipitation,and inhibition of insecticides were also studied.The specific activity of the extr |
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| Keywords: | fresh water fish acetylcholinesterase fractional precipitation insecticides susceptibility |
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