| 菊花离体叶片快繁体系的建立 |
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| 引用本文： | 林青萍,陈雄庭.菊花离体叶片快繁体系的建立[J].热带农业科学,2006,26(3):25-28. |
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| 作者姓名： | 林青萍 陈雄庭 |
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| 作者单位： | 热带作物生物技术国家重点实验室中国热带农业科学院热带生物技术研究所 海口市571101 |
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| 摘 要： | 以菊花叶片为外植体,诱导植株再生,建立高效植株再生体系。结果在MS 2mg/LBA 0.2mg/LNAA的培养基上诱导出愈伤组织,在MS 3mg/LBA 0.1mg/LNAA的培养基上诱导出芽,并在1/2MS 0.6mg/LNAA的培养基上诱导出根,从而获得再生完整植株,生根的小苗在温室生长良好。
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| 关 键 词： | 菊花 外植体 再生体系 |
| 收稿时间： | 2006-02-13 |
| 修稿时间： | 2006年2月13日 |
Establishment of Efficient Plant Regeneration System from Leaves of Chrysanthemum |
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| LIN Qingping CHEN Xiongting.Establishment of Efficient Plant Regeneration System from Leaves of Chrysanthemum[J].Chinese Journal of Tropical Agriculture,2006,26(3):25-28. |
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| Authors: | LIN Qingping CHEN Xiongting |
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| Institution: | State Key Biotechnology Laboratory for Tropical Crops / Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou 571101, Hainan |
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| Abstract: | A plant regeneration system from leaves of chrysanthemum was developed in this study. The optimum media were obtained for callus induction, shoot regeneration and rooting. Calli were induced from leaf explants cultured on the medium MS BA 2 mg/L NAA 0.2 mg/L. Bud shoots were induced from the calli cultured on the medium MS BA 3 mg/L NAA 0.1 mg/L, and rooted on the half-strength MS medium at the presence of BA 0.6 mg/L. The rooted plantlets survived in greenhouse. |
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| Keywords: | chrysanthemum explants plant regeneration system |
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